Reviewed, UniProtKB/Swiss-Prot Q89A99 (LON_BUCBP)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Ordered Locus Names:	bbp_421

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Degrades the regulatory proteins rcsA and sulA. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 780	780	ATP-dependent protease La		PRO_0000076127
Regions
	Domain	10 – 202	193	Lon
	Nucleotide binding	356 – 363	8	ATP Potential
Sites
	Active site	679	1	By similarity
	Active site	722	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89A99-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2C0AF017E21E6EAA	FASTA	780	88,203
	10 20 30 40 50 60 MNPERSEYIE IPVLPLRDVV IYPYMVIPLF VGRDKSIKCI EASMNKNKKI MLVTQKEAEI 70 80 90 100 110 120 DEPTDNDLFT IGTTASILQM LKLPDGTVKV LVEGLQRAKV KKINNENGYF TAQIQLICTP 130 140 150 160 170 180 EITEKEQSIL IRTTLNQFEN YVKFNKKISP EILNSLNNIT NASQLSDMIA IHMPLKLSEK 190 200 210 220 230 240 QSILETYNTN ERLERLMAIM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE 250 260 270 280 290 300 LGEMDETLDE HEILKRKITT IKMPKEAQEK MASELHKLKM MSPMSAEATV VRSYIDWMIQ 310 320 330 340 350 360 IPWNIRSKVK KNLTQAQKIL DSDHFGLDKV KERILEYLAV QSRINTVKGP ILCLVGPPGV 370 380 390 400 410 420 GKTSLGQSIA RATGRKYIRM ALGGIRDEAE IRGHRRTYIG SMPGKIIQKM AKVGVKNPLF 430 440 450 460 470 480 LLDEIDKMSC DIRVDPASAL LEVLDPEQNV AFNDHYLEVD YDLSNVMFIA TSNSTNIPAP 490 500 510 520 530 540 LLDRMEIIRI SGYTEFEKLN IAKSYLKPKQ IKRNALKSNE LTIEDSVVTN IIRYYTREAG 550 560 570 580 590 600 VRNLERELSK ICRKCVKNLI LNKSLKKIKI TTDNLHDYLG IKKYDFGKTN YKNQIGQVIG 610 620 630 640 650 660 LAWTEVGGEL LTIEAACISG KGKLIYTGSL GEVMQESIQA ALTVVRSQAN KLGIKKNFYE 670 680 690 700 710 720 KNDIHVHVPE GATPKDGPSA GIAMCTAIVS CLTGIPVKSD IAMTGEITLR GQILTIGGLK 730 740 750 760 770 780 EKLLAAHRGG IKKVLIPYDN EKDLQEIPKT ILKGLFVHPV KHIKEVLNLS LENTPYFSSK

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO27131.1.
RefSeq	NP_778026.1.
3D structure databases
SMR	Q89A99. Positions 491-584, 594-775.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Genome annotation databases
GeneID	1058354.
GenomeReviews	Gene locus bbp_421 in contig AE016826_GR.
KEGG	bab:bbp421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89A99.
OMA	Q89A99. VMKESIQ.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_421-MON.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_BUCBP

Accession

Primary (citable) accession number: Q89A99

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)