Q89A99 (LON_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	bbp_421

Organism

Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]

Taxonomic identifier

224915 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 780	780	Lon protease		PRO_0000076127
Regions
	Domain	10 – 201	192	Lon
	Nucleotide binding	356 – 363	8	ATP By similarity
Sites
	Active site	679	1	By similarity
	Active site	722	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89A99 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2C0AF017E21E6EAA	FASTA	780	88,203
	10 20 30 40 50 60 MNPERSEYIE IPVLPLRDVV IYPYMVIPLF VGRDKSIKCI EASMNKNKKI MLVTQKEAEI 70 80 90 100 110 120 DEPTDNDLFT IGTTASILQM LKLPDGTVKV LVEGLQRAKV KKINNENGYF TAQIQLICTP 130 140 150 160 170 180 EITEKEQSIL IRTTLNQFEN YVKFNKKISP EILNSLNNIT NASQLSDMIA IHMPLKLSEK 190 200 210 220 230 240 QSILETYNTN ERLERLMAIM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE 250 260 270 280 290 300 LGEMDETLDE HEILKRKITT IKMPKEAQEK MASELHKLKM MSPMSAEATV VRSYIDWMIQ 310 320 330 340 350 360 IPWNIRSKVK KNLTQAQKIL DSDHFGLDKV KERILEYLAV QSRINTVKGP ILCLVGPPGV 370 380 390 400 410 420 GKTSLGQSIA RATGRKYIRM ALGGIRDEAE IRGHRRTYIG SMPGKIIQKM AKVGVKNPLF 430 440 450 460 470 480 LLDEIDKMSC DIRVDPASAL LEVLDPEQNV AFNDHYLEVD YDLSNVMFIA TSNSTNIPAP 490 500 510 520 530 540 LLDRMEIIRI SGYTEFEKLN IAKSYLKPKQ IKRNALKSNE LTIEDSVVTN IIRYYTREAG 550 560 570 580 590 600 VRNLERELSK ICRKCVKNLI LNKSLKKIKI TTDNLHDYLG IKKYDFGKTN YKNQIGQVIG 610 620 630 640 650 660 LAWTEVGGEL LTIEAACISG KGKLIYTGSL GEVMQESIQA ALTVVRSQAN KLGIKKNFYE 670 680 690 700 710 720 KNDIHVHVPE GATPKDGPSA GIAMCTAIVS CLTGIPVKSD IAMTGEITLR GQILTIGGLK 730 740 750 760 770 780 EKLLAAHRGG IKKVLIPYDN EKDLQEIPKT ILKGLFVHPV KHIKEVLNLS LENTPYFSSK

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27131.1.
RefSeq	NP_778026.1. NC_004545.1.
3D structure databases
ProteinModelPortal	Q89A99.
SMR	Q89A99. Positions 9-117, 491-584, 594-775.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002268; EBBUCP00000002092; EBBUCG00000002268.
GeneID	1058354.
GenomeReviews	Gene locus bbp_421 in contig AE016826_GR.
KEGG	bab:bbp421.
PATRIC	21245659. VBIBucAph80364_0422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007957.
HOGENOM	HBG566281.
OMA	FMEGEIS.
PhylomeDB	Q89A99.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_421-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
KO	K01338.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_BUCBP

Accession

Primary (citable) accession number: Q89A99

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program