Reviewed, UniProtKB/Swiss-Prot Q89A96 (MDLB_BUCBP)

Names and origin

Protein names

Recommended name:
    Multidrug resistance-like ATP-binding protein mdlB
    EC=3.6.3.44

Gene names

Name:	mdlB
Ordered Locus Names:	bbp_424

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Multidrug resistance-like ATP-binding protein mdlB		PRO_0000092501
Regions
	Transmembrane	26 – 46	21	Potential
	Transmembrane	59 – 79	21	Potential
	Transmembrane	143 – 163	21	Potential
	Transmembrane	166 – 186	21	Potential
	Transmembrane	196 – 216	21	Potential
	Transmembrane	260 – 280	21	Potential
	Domain	25 – 308	284	ABC transmembrane type-1
	Domain	339 – 573	235	ABC transporter
	Nucleotide binding	373 – 380	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89A96-1 [UniParc]. Last modified May 30, 2003. Version 1. Checksum: 3B84848CE196ADF7	FASTA	578	66,827
	10 20 30 40 50 60 MNNVIDFWPT LKRLLYYGTN VKKYLILGFT LLLFSSIFEV LNPILISCFI KHYFINNTVN 70 80 90 100 110 120 YSLKIITYYL ILQILAAILN YHQNIIFNKI SLTVIQKLRY DVMSSTLQLP IKMFDQRPIG 130 140 150 160 170 180 QFISRITNDT ETIKELYDTV IKSLFQNIIL ILITLITMFI LEWRMACIAS IIFPIALIIM 190 200 210 220 230 240 LLYQYFSKPI LRKIKVYIAN IYNIFNEIIN GIDVIQQFHQ EQKFRKSIKK ISISHYYFRM 250 260 270 280 290 300 KILKLDSFLL RPLLNFCSTL ILCGLILIFG IYPIGFFEIG TLYAFITYLN RLNEPLITIT 310 320 330 340 350 360 SQQSIFQQAI VAGERIFEII HTPKQQYGDD SLHFREGNIK VKNLYFSYTN NNVYVLKNIN 370 380 390 400 410 420 IFIPSKQFIA FVGRTGSGKS TLSKLLIGHY PATLGKICLD ERNIQTFTHN VLKKNISIVQ 430 440 450 460 470 480 QDPIILNDTI LENITLGRNI STKKVLKILK TIKLIQFVNS LPKGLKTLLG ENGNILSIGQ 490 500 510 520 530 540 KQLLSIARTL ISCPKILILD EATSNVDLDT ENNIKKILSS VKHLTTIIAI THRLSTIKHA 550 560 570 DNIFVFNNGE IVESGTHYNL IRKKSYYKNM YYSQAIKN

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27134.1.
RefSeq	NP_778029.1.
3D structure databases
SMR	Q89A96. Positions 11-575.
ModBase	Search...
Genome annotation databases
GeneID	1058630.
GenomeReviews	Gene locus bbp_424 in contig AE016826_GR.
KEGG	bab:bbp424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758042.
OMA	KISISHY.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_424-MONOMER.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. False negative. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDLB_BUCBP

Accession

Primary (citable) accession number: Q89A96

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2003
Last sequence update:	May 30, 2003
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)