Reviewed, UniProtKB/Swiss-Prot Q89A46 (TRMB_BUCBP)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	bbp_499

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000171309
Regions
	Region	214 – 217	4	Substrate binding Potential
Sites
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	91	1	S-adenosyl-L-methionine By similarity
	Binding site	118	1	S-adenosyl-L-methionine By similarity
	Binding site	141	1	S-adenosyl-L-methionine By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	177	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89A46-1 [UniParc]. Last modified June 16, 2003. Version 1. Checksum: 9AAF0C3D4D2879A0	FASTA	242	28,732
	10 20 30 40 50 60 MYFYLKYNIN NMIMRRVRSF VSRNRKLTQN KRQFLKNYLL KYGIDFSCSY VNFNFIFNNE 70 80 90 100 110 120 YPVILEIGFG TGEFLINMAR KNLFSNFLGI EVYIPSILSC LRYIHKYNLP NVKVIFYDAV 130 140 150 160 170 180 EVISYMISNN SLSEVYILFP DPWPKRRHHK RRIITKELLE VILKKLVFGG YLNIATDCQI 190 200 210 220 230 240 YAKSILNIIE NIKGYINLSN TGDYVIRSDY RLVTKFEKKG LVLGNKIFNL KFKSIFNNSN FL

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO27204.1.
RefSeq	NP_778099.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1058500.
GenomeReviews	Gene locus bbp_499 in contig AE016826_GR.
KEGG	bab:bbp499.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89A46.
OMA	Q89A46. YAEWIDE.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_499-MON.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_BUCBP

Accession

Primary (citable) accession number: Q89A46

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)