PTS system mannitol-specific EIICBA component - Buchnera aphidicola subsp. Baizongia pistaciae

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Reviewed, UniProtKB/Swiss-Prot Q89A36 (PTM3C_BUCBP)

Last modified February 9, 2010. Version 54. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICBA component
Alternative name(s):
    EIICBA-Mtl
      Short name=EII-Mtl
Including the following 3 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component
    3- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system mannitol-specific EIIA component

Gene names

Name:	mtlA
Ordered Locus Names:	bbp_517

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

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Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Post-translational modification	An intramolecular phosphotransfer takes places between His-556 and Cys-385 By similarity.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

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Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 640

640

PTS system mannitol-specific EIICBA component

PRO_0000186613

Regions

Transmembrane

18 – 38

Potential

Transmembrane

52 – 72

Potential

Transmembrane

83 – 103

Potential

Transmembrane

134 – 154

Potential

Transmembrane

161 – 181

Potential

Transmembrane

186 – 206

Potential

Transmembrane

213 – 233

Potential

Transmembrane

269 – 289

Potential

Transmembrane

313 – 333

Potential

Domain

12 – 343

332

PTS EIIC type-2

Domain

379 – 475

PTS EIIB type-2

Domain

496 – 638

143

PTS EIIA type-2

Sites

Active site

385

Phosphocysteine intermediate; for EIIB activity By similarity

Active site

556

Tele-phosphohistidine intermediate; for EIIA activity By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q89A36-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: A5B76D06E6909C3B
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FASTA

640

70,759

        10         20         30         40         50         60 
MSVPMTVKVQ NLGRFLSAMI MPNISVFIAW GIISGLFIKS GWCPNQTLEK VLSPISVYLF 

        70         80         90        100        110        120 
PILIANTGGY LINGKRGAIV GSIAVVGAII STAIPMLLGA MIIGPIGGWI TYYFDKISKY 

       130        140        150        160        170        180 
KVKSGFEMLV NNFSVGILGV LLLFISFLCI GPMIEKLSCF LGYVVNLMIN NHLLPFIAIL 

       190        200        210        220        230        240 
IEPAKIFFLN NVINHGVLFP LGIQEVVKFN KSIFFLIESN PGPGIGVLMA WFFFGCDNIK 

       250        260        270        280        290        300 
KSLKEAIVIQ LFGGIHEIYF PYVLKNPRLI LALILGSITG IFILIVLRGG LISAASPGSI 

       310        320        330        340        350        360 
ISILAMTPKG LYVINLLAII ISFLVSFLVS CMLLKISNRN HYVKGSNTKI EKDNFLINSS 

       370        380        390        400        410        420 
FQKNRTCIKS SLDSHKCIRN IIFACDAGMG SSAVAAGILR NKIHDLNIFN ITVSNAAIDS 

       430        440        450        460        470        480 
IPNFGVDLII THYSLTDRAR KRNSNAKHLS LNSFLDNAFY NELSKYLVEN NLDNNSSILD 

       490        500        510        520        530        540 
FSVRNQNNFS SKKNVFSLTK ENIFLGQIAS SKEEVIRFIG RQLVNQGYVK EEYIEAMLER 

       550        560        570        580        590        600 
EKMMSTWLGE SIALPHGTIQ SKDFILNTGI IFCQFPNGIL FGDDPEDIAH LVIGVAARNN 

       610        620        630        640 
EHIPVVSNIT NILDNNDVIK SLSITKNIDD VLYLFSRKNI

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References

[1]

"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016826 Genomic DNA. Translation: AAO27220.1.
RefSeq	NP_778115.1.
3D structure databases
SMR	Q89A36. Positions 378-471, 496-638.
ModBase	Search...
Genome annotation databases
GeneID	1058455.
GenomeReviews	Gene locus bbp_517 in contig AE016826_GR.
KEGG	bab:bbp517.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296680.
OMA	KSARTAN.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_517-MONOMER.
Family and domain databases
InterPro	IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

PTM3C_BUCBP

Accession

Primary (citable) accession number: Q89A36

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents