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Q89A30 (TRPG_BUCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anthranilate synthase component 2

Short name=AS
Short name=ASII
EC=4.1.3.27
Alternative name(s):
Anthranilate synthase, GATase component
Anthranilate synthase, glutamine amidotransferase component
Gene names
Name:trpG
Ordered Locus Names:bbp_525
OrganismBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp) [Complete proteome] [HAMAP]
Taxonomic identifier224915 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length192 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia By similarity.

Catalytic activity

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.

Subunit structure

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE) By similarity.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 192192Anthranilate synthase component 2
PRO_0000056874

Regions

Domain3 – 192190Glutamine amidotransferase type-1
Region57 – 593Glutamine binding By similarity
Region134 – 1352Glutamine binding By similarity

Sites

Active site841For GATase activity By similarity
Active site1701For GATase activity By similarity
Active site1721For GATase activity By similarity
Binding site841Glutamine By similarity
Binding site881Glutamine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89A30 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: BBE006C1FC39B0BC

FASTA19221,603
        10         20         30         40         50         60 
MGNILLLDNI DSFTYNLVDQ LRSNFHQVFV YRNTVRKNII LKKLSKMINP ILILSPGPGN 

        70         80         90        100        110        120 
PDNAGCMPQL LMELKGKLPI IGICLGHQAI VKMYGGHVGY SGEILHGQAS LINHDNKAMF 

       130        140        150        160        170        180 
LGMSNPLPVA RYHSLICSNI PNMLVVNAHF NNMVMAVRND YEKICGFQFH PESILTTRGT 

       190 
EFLQRVIQWT KI 

« Hide

References

[1]"Reductive genome evolution in Buchnera aphidicola."
van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.
Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016826 Genomic DNA. Translation: AAO27227.1.
RefSeqNP_778122.1. NC_004545.1.

3D structure databases

ProteinModelPortalQ89A30.
SMRQ89A30. Positions 2-189.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224915.bbp525.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO27227; AAO27227; bbp_525.
GeneID1058501.
KEGGbab:bbp525.
PATRIC21245873. VBIBucAph80364_0521.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0512.
KOK01658.
OMAANPMPVA.
OrthoDBEOG6M9F11.

Enzyme and pathway databases

BioCycBAPH224915:GJ9D-525-MONOMER.
UniPathwayUPA00035; UER00040.

Family and domain databases

Gene3D3.40.50.880. 1 hit.
InterProIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamPF00117. GATase. 1 hit.
[Graphical view]
SUPFAMSSF52317. SSF52317. 1 hit.
TIGRFAMsTIGR00566. trpG_papA. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPG_BUCBP
AccessionPrimary (citable) accession number: Q89A30
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways