Reviewed, UniProtKB/Swiss-Prot Q89A20 (ILVC_BUCBP)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	bbp_541

Organism

Buchnera aphidicola subsp. Baizongia pistaciae [Complete proteome] [HAMAP]

Taxonomic identifier

135842 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Ketol-acid reductoisomerase		PRO_0000151288
Sites
	Active site	133	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89A20-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9583C462A6D0EA82	FASTA	491	55,968
	10 20 30 40 50 60 MGSNYFNSLN FRQKLIQLRQ CTLIRKEEFS ETFKILKGKN IVIVGCGSQG LNQGLNMRDS 70 80 90 100 110 120 GLNVKYALRK NSIISQRSSW KRAIDNKFLV GTCEELIPTA DLVINLTPDK QHEHVVDVLQ 130 140 150 160 170 180 NLMKKNSILG FSHGFNIIEF GQKIRQDITV IMVAPKCPGT EVREEYKRNF GVPTLISVHL 190 200 210 220 230 240 DNDCHNIGLE VAKEWATAIG GHRAGVLHSS FSAEVKSDLM GEQTILCGLL QTGSVLCYDK 250 260 270 280 290 300 LISQGHDCHY AVKLVQSGWE VITESLKHGG ITLMFDRLSN TAKIRAYKLS NDLKKILSPI 310 320 330 340 350 360 FKKHMDDILS GYYSKNMMLD WKNDDKQLKI WRRSIQNTSF EQCSISHITL LEHEYFENGL 370 380 390 400 410 420 LMVAIIRAGV ELAFEIMIEA GIKEESAYYE SLHELPLIAN TIARKRLYEM NLIISDTAEY 430 440 450 460 470 480 GSHLFFQSAF PLLQDFMNTL EHGDLGNKML DQAINNVDLN YINTTIRNHP VEHIGQKLRR 490 YMTNIKQINC Y

References

[1]

"Reductive genome evolution in Buchnera aphidicola." van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F., Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J., Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A. Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003) [PubMed: 12522265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016826 Genomic DNA. Translation: AAO27240.1.
RefSeq	NP_778135.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	1058333.
GenomeReviews	Gene locus bbp_541 in contig AE016826_GR.
KEGG	bab:bbp541.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89A20.
Enzyme and pathway databases
BioCyc	BAPH224915:BBP_541-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BUCBP

Accession

Primary (citable) accession number: Q89A20

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)