Q899S1 (MNMG_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	CTC_00099

Organism

Clostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]

Taxonomic identifier

212717 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium tetani

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	oxidation-reduction process Inferred from electronic annotation. Source: InterPro tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129		PRO_0000117089
Regions
	Nucleotide binding	14 – 19	6	FAD By similarity
	Nucleotide binding	273 – 287	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q899S1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C9F03133AFAD667C	FASTA	623	69,943
	10 20 30 40 50 60 MKYYAGDYDV VVVGAGHAGC EAALAAARIG CKVLICTISL DSVALMPCNP NIGGTAKGHL 70 80 90 100 110 120 VREIDALGGE MGVNIDNTFI QSRMLNTSKG PAVHSLRAQA DKSRYSNRMK YILENEENVT 130 140 150 160 170 180 LKQIEVISID IENGKVKGIL TKNGAYYNAK TVVLATGTYL NARIIIGEVA YSGGPNGLFP 190 200 210 220 230 240 AKELTKNLMD LGISIRRFKT GTPARVNKKT IDFSKMIEQP GDEKIVPFSF LTDKLEREQV 250 260 270 280 290 300 SCYLTYTNEN THEVIRKNLH RSPMFNGSIE GVGARYCPSI EDKVNRFPDK NKHQVFIEPE 310 320 330 340 350 360 GEYTNEMYVS GLSSSLPEEI QVAMYRTVPG LENVEFLRTA YAIEYDCIDP QELKLSLESK 370 380 390 400 410 420 NIEGLFSGGQ INGSSGYEEA AAQGLIAGIN AAMKVKEKDP LLLTRSDGYI GVLIDDLVTK 430 440 450 460 470 480 GTNEPYRMMT SRSEYRLLLR QGNADLRLTQ KGYDVGLVSE ERYKRYINRR ESIKSEIERI 490 500 510 520 530 540 KNVQITNKKE VNEFLLSLNS SELKKPISLY ELIKRPELDY YKVEQLDKGR INLPEDVQEE 550 560 570 580 590 600 VNTTAKYEGY IEKQLEQVNQ FKKFENKLLP NDIDYSKVYG LRIESVQKLS KIRPMNIGQA 610 620 SRISGVSPAD ISVLLIYLEH KYK

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO34751.1.
RefSeq	NP_780814.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q899S1.
ModBase	Search...
Protein-protein interaction databases
STRING	212717.CTC00099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO34751; AAO34751; CTC_00099.
GeneID	1058794.
KEGG	ctc:CTC00099.
PATRIC	19507940. VBICloTet101274_0046.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0445.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	CTET212717:GJAM-51-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_CLOTE

Accession

Primary (citable) accession number: Q899S1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program