Reviewed, UniProtKB/Swiss-Prot Q899P3 (FABH_CLOTE)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3
    EC=2.3.1.180
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III
    Beta-ketoacyl-ACP synthase III
      Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	CTC_00127

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity.
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity.
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_0000110420
Regions
	Region	261 – 265	5	ACP-binding By similarity
Sites
	Active site	114	1	By similarity
	Active site	260	1	By similarity
	Active site	290	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q899P3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0640A0664E3D5FDF	FASTA	334	36,699
	10 20 30 40 50 60 MKNKEVRILS TGKYLPPISI SNHDLSKIID TNDNWIKTRT GIEKRRITKG ENTSDLGTKA 70 80 90 100 110 120 ALDALRKGGI SPEELDLIIV ATITPDYFTP STACIIQRNI KAYNAFAFDI SAACSGFTYG 130 140 150 160 170 180 ISIASQFIRN GVAKKVLVIG VETLSKLVDW KDRNTCILFG DGSGAAILTE SNEKGIMNVY 190 200 210 220 230 240 LGSDGRGADL LKCKSSSLTV NSDELKELLN SKEEDLENKF IEMDGKEIFK FAVKVMIKGI 250 260 270 280 290 300 EKVLKDSNLE LKDINYIIPH QANLRIIEHV AKKLGIDENK FYININHYGN TSAASIPIAL 310 320 330 AEVDEKGLLK KGDNVILVGF GAGLTWAASL IKWI

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO34779.1.
RefSeq	NP_780842.1.
3D structure databases
HSSP	HSSP built from PDB template 1HNK based on UniProtKB P24249.
ModBase	Search...
Genome annotation databases
GeneID	1059174.
GenomeReviews	Gene locus CTC_00127 in contig AE015927_GR.
KEGG	ctc:CTC00127.
NMPDR	fig|212717.1.peg.64.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q899P3.
OMA	YIVPHQA.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_00127-MON.
BRENDA	2.3.1.180. 2082.
Family and domain databases
HAMAP	MF_01815. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_CLOTE

Accession

Primary (citable) accession number: Q899P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)