Reviewed, UniProtKB/Swiss-Prot Q899M9 (SYN_CLOTE)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	CTC_00143

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176403

Sequences

	Sequence		Length	Mass (Da)
	Q899M9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5910CF90B2393529	FASTA	463	53,370
	10 20 30 40 50 60 MKKISIKQLY REPEKFINKD IVVAGWIRTE RTSKNFGFIE LNDGSFFKNL QIIIDTNLDN 70 80 90 100 110 120 FDKVSKLPIS SSIVVEGKLV ATPSAKQPFE VQASKVTVEG TSLQDYPLQK KRHSFEFLRS 130 140 150 160 170 180 IAHLRPRSNT FSAVFRIRSL AAYAVHKFFQ EKNFVYVHTP IITASDCEGA GEMFRVTTLD 190 200 210 220 230 240 LNNIPKNDEG KIDFSQDFFD KDAKLTVSGQ LSAESYALAF GNVYTFGPTF RAENSNTARH 250 260 270 280 290 300 ASEFWMIEPE MAFADLTDYM DVAEEMVKYI INYVLENAPE EMNFFNSFID KDLFKRLDNV 310 320 330 340 350 360 VNSEFKRITY TEAVDLLQKS GEKFQYPVEW GIDLQTEHER YITEKIFGKP VFVTDYPKDI 370 380 390 400 410 420 KAFYMRLNED NKTVAAADLL VPGVGEIIGG SQREERLDVL EERMKEFDLN EKDYWWYLEL 430 440 450 460 RKYGSAKHSG FGLGFERILM YMTGISNIRD VIPFPRTPGS AEF

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO34795.1.
RefSeq	NP_780858.1.
3D structure databases
HSSP	HSSP built from PDB template 1B8A based on UniProtKB Q52428.
ModBase	Search...
Genome annotation databases
GeneID	1059195.
GenomeReviews	Gene locus CTC_00143 in contig AE015927_GR.
KEGG	ctc:CTC00143.
NMPDR	fig|212717.1.peg.80.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q899M9.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_00143-MON.
BRENDA	6.1.1.22. 2082.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CLOTE

Accession

Primary (citable) accession number: Q899M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)