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Protein

Biotin synthase

Gene

bioB

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi71Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi74Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi111Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi143Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi203Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi273Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCTET212717:G1G04-122-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:CTC_00151
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001412 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003813221 – 325Biotin synthaseAdd BLAST325

Proteomic databases

PRIDEiQ899M1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC00151

Structurei

3D structure databases

ProteinModelPortaliQ899M1
SMRiQ899M1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q899M1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIILEIEE KIYSDEDITF NEAITLFQSS YDKNLNNLLD AANRIRNKFN
60 70 80 90 100
GNIVDLCSIM NAKSGRCSED CKFCAQSEHY NTNIKKYNMI NMSDALNLAK
110 120 130 140 150
ENKEHGINRF SLVTSGKALT SNDFEKALEI YKEINKRVNI NLCASLGILS
160 170 180 190 200
YNQLLQLKNV GVTMYHHNLE TSREYYNKIC TTHSYDERIE TINNAKKAGL
210 220 230 240 250
MVCSGGIIGM GETITDRIKL AFELKSLQIQ SIPINILNPV KGTPLEYTKR
260 270 280 290 300
LEQNDILKTI AIFRFINPKA SIRLAGGRNL IDNFGKGCFR AGANATISGN
310 320
YLTTSGNKIN DDIEMIKSLG LSLDI
Length:325
Mass (Da):36,521
Last modified:June 1, 2003 - v1
Checksum:iC2B1A81CA60C5003
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA Translation: AAO34803.1
RefSeqiWP_011098475.1, NC_004557.1

Genome annotation databases

EnsemblBacteriaiAAO34803; AAO34803; CTC_00151
GeneIDi24253745
KEGGictc:CTC_00151

Similar proteinsi

Entry informationi

Entry nameiBIOB_CLOTE
AccessioniPrimary (citable) accession number: Q899M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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