Reviewed, UniProtKB/Swiss-Prot Q899I8 (KPRS_CLOTE)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Ordered Locus Names:	CTC_00188

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity HAMAP MF_00583.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141128
Regions
	Region	220 – 233	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q899I8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9FA189C1D888A720	FASTA	320	34,985
	10 20 30 40 50 60 MVMIHERKSL KIFAGNSNDK LAKDIADIVG VPLGSAEVGR FSDGEITVNI NETVRGIDAF 70 80 90 100 110 120 IIQSTSAPVN DNLMELLIMI DAFKRASAGR ITAVIPYYGY ARQDRKTKAR DPITAKLVAD 130 140 150 160 170 180 ILTAAGADRV LTMDLHASQI QGYFDIPLDH LLGVPILGKY FAENGFDQRE DVVVVSPDLG 190 200 210 220 230 240 SVTRARKFAD RIHAPIAIID KRRPKPNVSE VMHIIGDIKD KTCILIDDMI DTAGTITNGA 250 260 270 280 290 300 NALVERGAKD VYACCTHAVL SGPAMERIDN SVIKELITLN TIDISKIKPS DKIKVLSVAP 310 320 VFAEAIRRIY EEISVSKLFD

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO34838.1.
RefSeq	NP_780901.1.
3D structure databases
SMR	Q899I8. Positions 8-319.
ModBase	Search...
Genome annotation databases
GeneID	1059268.
GenomeReviews	Gene locus CTC_00188 in contig AE015927_GR.
KEGG	ctc:CTC00188.
NMPDR	fig|212717.1.peg.123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG519284.
OMA	CATHAVF.
PhylomeDB	Q899I8.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_00188-MONOMER.
BRENDA	2.7.6.1. 2082.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_CLOTE

Accession

Primary (citable) accession number: Q899I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)