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Q899C3

- SYI_CLOTE

UniProt

Q899C3 - SYI_CLOTE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei593 – 5931ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCTET212717:GJAM-221-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CTC_00261
    OrganismiClostridium tetani (strain Massachusetts / E88)
    Taxonomic identifieri212717 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001412: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10361036Isoleucine--tRNA ligasePRO_0000098535Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi212717.CTC00261.

    Structurei

    3D structure databases

    ProteinModelPortaliQ899C3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi590 – 5945"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiSEGMEPN.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q899C3-1 [UniParc]FASTAAdd to Basket

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    MYKKIDGSKS FVQMEREVLD FWNKNNIVDK SFALNEEGEY FTFYDGPPTA     50
    NGKPHVGHVL TRVIKDLIPR YKVMKGYKVL RKAGWDTHGL PVELEIEKKL 100
    GISGKEEIEK FGVEEFIKEC KDSVFTYSSM WKDMSEKLAF WVDMENPYVT 150
    YHNDYIESVW WALKQLWNKE LLYKGHKVIP YCPRCGTALS SHEVAQGYKD 200
    VKEATAFVKF KVKGEENKYI LAWTTTPWTL PSNVALAINK NFDYVEVKNN 250
    DEVLILAKEL VDSVIDGEYE IIKEFKGEDI LGLQYEQLLP FYTPEEEAFR 300
    VIHGDFVTLS DGTGIVHTAP AYGEDDNIVC KKHGLPMINL VDKEGKFIDC 350
    VEPWKGMPVK KADSKIIEYM DEKGILYKSE KFTHSYPHCW RCDTALLYYP 400
    TDSWFVRMTS LRDKLLENNN KVNWYPDNIR TGRFGKFLEN VIDWGISRDR 450
    YWGTPLPIWE CECGHRECIG SISELKEKGI DVPEDIELHK PYIDKVKLKC 500
    SKCGKEMKRT REVIDCWFDS GSMPFAQHHY PFENKEVFEK TFPAQFISEA 550
    VDQTRGWFYT LTAISTAIFD TNPFENCIVL GHVLDKHGLK MSKSKGNVVD 600
    PFDVLDSAGA DASRWHFYTA SAPWLPTRFS PEDVEETQRK FLSTLWNVYS 650
    FYVLYADIDK FNPLEYKDFV SENVMDKWIV SKLNSLIKDV EDHMDSYRIT 700
    QAALAIEDFV DELSNWYVRR NRSRFWSEEL REDKIGAYVT LYKVLTTVSL 750
    IAAPFVPFIT EEIYNNLVRG LDKNALESIH LCNWPKYDEN LIQKELEREM 800
    DEAYKIVKLG RSARNSVNIK NRQPLSSMLV SIKTLPEYYG RIIKDELNIK 850
    DIIFGADLSS YVEFNIKPNL PVLGKAYGRY IPQIRKEITS MDQMKLAQKI 900
    KQGEKVIINI DGNEIELNEE NLLVTMKGLE GFAFAGEGNI GVVLNTTITD 950
    ELKEEGQLRE ILSKIQNMRK EKEFEVADRI KLYVSGNRML ESVVEKFEDI 1000
    IRKETIAEEV IYNEEREYVD CKINGEDFKI EVEAIK 1036
    Length:1,036
    Mass (Da):120,322
    Last modified:December 20, 2005 - v2
    Checksum:i53261B634ADDD476
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015927 Genomic DNA. Translation: AAO34906.1.
    RefSeqiNP_780969.1. NC_004557.1.

    Genome annotation databases

    EnsemblBacteriaiAAO34906; AAO34906; CTC_00261.
    GeneIDi1059829.
    KEGGictc:CTC00261.
    PATRICi19508330. VBICloTet101274_0226.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015927 Genomic DNA. Translation: AAO34906.1 .
    RefSeqi NP_780969.1. NC_004557.1.

    3D structure databases

    ProteinModelPortali Q899C3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 212717.CTC00261.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO34906 ; AAO34906 ; CTC_00261 .
    GeneIDi 1059829.
    KEGGi ctc:CTC00261.
    PATRICi 19508330. VBICloTet101274_0226.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi SEGMEPN.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CTET212717:GJAM-221-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Massachusetts / E88.

    Entry informationi

    Entry nameiSYI_CLOTE
    AccessioniPrimary (citable) accession number: Q899C3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3