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Q899C3

- SYI_CLOTE

UniProt

Q899C3 - SYI_CLOTE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTET212717:GJAM-221-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CTC_00261
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001412: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligasePRO_0000098535Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC00261.

Structurei

3D structure databases

ProteinModelPortaliQ899C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi590 – 5945"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiSEGMEPN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q899C3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYKKIDGSKS FVQMEREVLD FWNKNNIVDK SFALNEEGEY FTFYDGPPTA
60 70 80 90 100
NGKPHVGHVL TRVIKDLIPR YKVMKGYKVL RKAGWDTHGL PVELEIEKKL
110 120 130 140 150
GISGKEEIEK FGVEEFIKEC KDSVFTYSSM WKDMSEKLAF WVDMENPYVT
160 170 180 190 200
YHNDYIESVW WALKQLWNKE LLYKGHKVIP YCPRCGTALS SHEVAQGYKD
210 220 230 240 250
VKEATAFVKF KVKGEENKYI LAWTTTPWTL PSNVALAINK NFDYVEVKNN
260 270 280 290 300
DEVLILAKEL VDSVIDGEYE IIKEFKGEDI LGLQYEQLLP FYTPEEEAFR
310 320 330 340 350
VIHGDFVTLS DGTGIVHTAP AYGEDDNIVC KKHGLPMINL VDKEGKFIDC
360 370 380 390 400
VEPWKGMPVK KADSKIIEYM DEKGILYKSE KFTHSYPHCW RCDTALLYYP
410 420 430 440 450
TDSWFVRMTS LRDKLLENNN KVNWYPDNIR TGRFGKFLEN VIDWGISRDR
460 470 480 490 500
YWGTPLPIWE CECGHRECIG SISELKEKGI DVPEDIELHK PYIDKVKLKC
510 520 530 540 550
SKCGKEMKRT REVIDCWFDS GSMPFAQHHY PFENKEVFEK TFPAQFISEA
560 570 580 590 600
VDQTRGWFYT LTAISTAIFD TNPFENCIVL GHVLDKHGLK MSKSKGNVVD
610 620 630 640 650
PFDVLDSAGA DASRWHFYTA SAPWLPTRFS PEDVEETQRK FLSTLWNVYS
660 670 680 690 700
FYVLYADIDK FNPLEYKDFV SENVMDKWIV SKLNSLIKDV EDHMDSYRIT
710 720 730 740 750
QAALAIEDFV DELSNWYVRR NRSRFWSEEL REDKIGAYVT LYKVLTTVSL
760 770 780 790 800
IAAPFVPFIT EEIYNNLVRG LDKNALESIH LCNWPKYDEN LIQKELEREM
810 820 830 840 850
DEAYKIVKLG RSARNSVNIK NRQPLSSMLV SIKTLPEYYG RIIKDELNIK
860 870 880 890 900
DIIFGADLSS YVEFNIKPNL PVLGKAYGRY IPQIRKEITS MDQMKLAQKI
910 920 930 940 950
KQGEKVIINI DGNEIELNEE NLLVTMKGLE GFAFAGEGNI GVVLNTTITD
960 970 980 990 1000
ELKEEGQLRE ILSKIQNMRK EKEFEVADRI KLYVSGNRML ESVVEKFEDI
1010 1020 1030
IRKETIAEEV IYNEEREYVD CKINGEDFKI EVEAIK
Length:1,036
Mass (Da):120,322
Last modified:December 20, 2005 - v2
Checksum:i53261B634ADDD476
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015927 Genomic DNA. Translation: AAO34906.1.
RefSeqiNP_780969.1. NC_004557.1.

Genome annotation databases

EnsemblBacteriaiAAO34906; AAO34906; CTC_00261.
GeneIDi1059829.
KEGGictc:CTC00261.
PATRICi19508330. VBICloTet101274_0226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015927 Genomic DNA. Translation: AAO34906.1 .
RefSeqi NP_780969.1. NC_004557.1.

3D structure databases

ProteinModelPortali Q899C3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 212717.CTC00261.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO34906 ; AAO34906 ; CTC_00261 .
GeneIDi 1059829.
KEGGi ctc:CTC00261.
PATRICi 19508330. VBICloTet101274_0226.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi SEGMEPN.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTET212717:GJAM-221-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Massachusetts / E88.

Entry informationi

Entry nameiSYI_CLOTE
AccessioniPrimary (citable) accession number: Q899C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3