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Q899C3

- SYI_CLOTE

UniProt

Q899C3 - SYI_CLOTE

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Protein
Isoleucine--tRNA ligase
Gene
ileS, CTC_00261
Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTET212717:GJAM-221-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CTC_00261
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001412: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098535Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC00261.

Structurei

3D structure databases

ProteinModelPortaliQ899C3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiSEGMEPN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q899C3-1 [UniParc]FASTAAdd to Basket

« Hide

MYKKIDGSKS FVQMEREVLD FWNKNNIVDK SFALNEEGEY FTFYDGPPTA     50
NGKPHVGHVL TRVIKDLIPR YKVMKGYKVL RKAGWDTHGL PVELEIEKKL 100
GISGKEEIEK FGVEEFIKEC KDSVFTYSSM WKDMSEKLAF WVDMENPYVT 150
YHNDYIESVW WALKQLWNKE LLYKGHKVIP YCPRCGTALS SHEVAQGYKD 200
VKEATAFVKF KVKGEENKYI LAWTTTPWTL PSNVALAINK NFDYVEVKNN 250
DEVLILAKEL VDSVIDGEYE IIKEFKGEDI LGLQYEQLLP FYTPEEEAFR 300
VIHGDFVTLS DGTGIVHTAP AYGEDDNIVC KKHGLPMINL VDKEGKFIDC 350
VEPWKGMPVK KADSKIIEYM DEKGILYKSE KFTHSYPHCW RCDTALLYYP 400
TDSWFVRMTS LRDKLLENNN KVNWYPDNIR TGRFGKFLEN VIDWGISRDR 450
YWGTPLPIWE CECGHRECIG SISELKEKGI DVPEDIELHK PYIDKVKLKC 500
SKCGKEMKRT REVIDCWFDS GSMPFAQHHY PFENKEVFEK TFPAQFISEA 550
VDQTRGWFYT LTAISTAIFD TNPFENCIVL GHVLDKHGLK MSKSKGNVVD 600
PFDVLDSAGA DASRWHFYTA SAPWLPTRFS PEDVEETQRK FLSTLWNVYS 650
FYVLYADIDK FNPLEYKDFV SENVMDKWIV SKLNSLIKDV EDHMDSYRIT 700
QAALAIEDFV DELSNWYVRR NRSRFWSEEL REDKIGAYVT LYKVLTTVSL 750
IAAPFVPFIT EEIYNNLVRG LDKNALESIH LCNWPKYDEN LIQKELEREM 800
DEAYKIVKLG RSARNSVNIK NRQPLSSMLV SIKTLPEYYG RIIKDELNIK 850
DIIFGADLSS YVEFNIKPNL PVLGKAYGRY IPQIRKEITS MDQMKLAQKI 900
KQGEKVIINI DGNEIELNEE NLLVTMKGLE GFAFAGEGNI GVVLNTTITD 950
ELKEEGQLRE ILSKIQNMRK EKEFEVADRI KLYVSGNRML ESVVEKFEDI 1000
IRKETIAEEV IYNEEREYVD CKINGEDFKI EVEAIK 1036
Length:1,036
Mass (Da):120,322
Last modified:December 20, 2005 - v2
Checksum:i53261B634ADDD476
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015927 Genomic DNA. Translation: AAO34906.1.
RefSeqiNP_780969.1. NC_004557.1.

Genome annotation databases

EnsemblBacteriaiAAO34906; AAO34906; CTC_00261.
GeneIDi1059829.
KEGGictc:CTC00261.
PATRICi19508330. VBICloTet101274_0226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015927 Genomic DNA. Translation: AAO34906.1 .
RefSeqi NP_780969.1. NC_004557.1.

3D structure databases

ProteinModelPortali Q899C3.
ModBasei Search...

Protein-protein interaction databases

STRINGi 212717.CTC00261.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO34906 ; AAO34906 ; CTC_00261 .
GeneIDi 1059829.
KEGGi ctc:CTC00261.
PATRICi 19508330. VBICloTet101274_0226.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi SEGMEPN.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTET212717:GJAM-221-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Massachusetts / E88.

Entry informationi

Entry nameiSYI_CLOTE
AccessioniPrimary (citable) accession number: Q899C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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