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Reviewed, UniProtKB/Swiss-Prot Q898Z5 (DDLB_CLOTE)

Last modified November 3, 2009. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    D-alanine--D-alanine ligase B
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase B
    D-Ala-D-Ala ligase B
Gene names
Name: ddlB
Ordered Locus Names: CTC_00291
OrganismClostridium tetani [Complete proteome] [HAMAP]
Taxonomic identifier1513 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

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Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Cell wall formation By similarity.

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358D-alanine--D-alanine ligase B HAMAP MF_00047
PRO_0000177810

Regions

Domain147 – 352206ATP-grasp
Nucleotide binding179 – 23456ATP By similarity

Sites

Metal binding3051Magnesium or manganese 1 By similarity
Metal binding3191Magnesium or manganese 1 By similarity
Metal binding3191Magnesium or manganese 2 By similarity
Metal binding3211Magnesium or manganese 2 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q898Z5-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 36E6E9FA2AF9B8F9

FASTA35840,536
        10         20         30         40         50         60 
MLLAQQLNLY ILKGEIIMKK IKIAIVFGGQ STEHEVSRNS VTSILKNINR DKYEICPIGI 

        70         80         90        100        110        120 
TKEGKWFQYT GDINNIKNGQ WEKDNKNKLE NGYNVLFNKE VELVFPVLHG LYGEDGTIQG 

       130        140        150        160        170        180 
LCKLVGLPCV GPGVLSSALC MDKIYTKYVL ENFKFKQANY VVVNKFEYEK NKEEIIKEVE 

       190        200        210        220        230        240 
KLQYDVFIKP ANSGSSVGIT KAHNKEELLK GLEEAFIHDK NVLVEEAINA REIEVAVLGN 

       250        260        270        280        290        300 
DDPKAAVPGE IIPAKEFYDY EAKYQNENSE LLIPANIDNI KQEEIKELAI KIYKLLGCSG 

       310        320        330        340        350 
LARVDFLMDK ESNEVYFNEV NTLPGFTKIS MYPKLWEASG KSYSALIDEL IELAINNK

« Hide

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References

[1]"The genome sequence of Clostridium tetani, the causative agent of tetanus disease."
Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Massachusetts / E88.

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Cross-references

Sequence databases

AE015927 Genomic DNA. Translation: AAO34934.1.
RefSeqNP_780997.1.

3D structure databases

HSSPHSSP built from PDB template 1EHI based on UniProtKB P71454.
ModBaseSearch...

Genome annotation databases

GeneID1059868.
GenomeReviewsGene locus CTC_00291 in contig AE015927_GR.
KEGGctc:CTC00291.
NMPDRfig|212717.1.peg.219.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ898Z5.
OMAGREIECG.

Enzyme and pathway databases

BioCycCTET212717:CTC_00291-MON.
BRENDA6.3.2.4. 2082.

Family and domain databases

HAMAPMF_00047.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR013817. Pre-ATP_grasp.
[Graphical view]
Gene3DG3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDDLB_CLOTE
AccessionPrimary (citable) accession number: Q898Z5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents