Q898X3 (MURA2_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
EC=2.5.1.7

Alternative name(s):
Enoylpyruvate transferase 2
UDP-N-acetylglucosamine enolpyruvyl transferase 2
Short name=EPT 2

Gene names

Name:	murA2
Ordered Locus Names:	CTC_00314

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231191
Sites
	Active site	118	1	Proton donor By similarity
Amino acid modifications
	Modified residue	118	1	2-(S-cysteinyl)pyruvic acid O-phosphothioketal By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q898X3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0F13EE4BA3F3C9D1	FASTA	417	44,777
	10 20 30 40 50 60 MNKIIVNGGK SLKGEVNINS AKNSVLPIIA ASILSGDKCI IENAPMLEDV FVISEVLKSI 70 80 90 100 110 120 SSEVDIDRDN NKIIIDTSNI CNSEPCSELV KKLRASFLIM GPMISRFGNF KISLPGGCNI 130 140 150 160 170 180 GTRPIDLHLK GLNALGADIN IGYGYVEAKA DKLKGNKIYL DFPSVGATEN LMMAAVLAEG 190 200 210 220 230 240 DTIIQNAAEE PEIEDLAKFL NSMGANIVGA GTDTINIIGV KDLKGVVHKP IYDRIEAGTF 250 260 270 280 290 300 MTAAAITRSK IKLNGVNEGH LRPIIAKLTE IGVKIDTNGD SMIVDGNHQL KPVDIKTMPY 310 320 330 340 350 360 PGFPTDMQAQ TMALLTSIEG TSIVTETIFE NRFMHATEMK RMGSNIKIDG RSAVIEGGNE 370 380 390 400 410 LTGCEVKATD LRAGAALILC GLVARGSTNV TDVYHIDRGY ANIEKKLQAL GAEIYRI

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO34956.1.
RefSeq	NP_781019.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q898X3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1059891.
GenomeReviews	Gene locus CTC_00314 in contig AE015927_GR.
KEGG	ctc:CTC00314.
NMPDR	fig|212717.1.peg.241.
PATRIC	19508440. VBICloTet101274_0281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG482701.
OMA	YTPIYDR.
PhylomeDB	Q898X3.
ProtClustDB	PRK09369.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_00314-MONOMER.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00790.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01072. MurA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_CLOTE

Accession

Primary (citable) accession number: Q898X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program