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Q898V2 (SYL_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CTC_00337
OrganismClostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]
Taxonomic identifier212717 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length812 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAO34977.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 812812Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152004

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif572 – 5765"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5751ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q898V2 [UniParc].

Last modified June 20, 2003. Version 1.
Checksum: 230DDDDC5F31CB2D

FASTA81293,037
        10         20         30         40         50         60 
MGNYGTNIDR KWQNKWEDSN LYHFDTNNLD KKLYVLEMFS YPSGSNLHAG HWFNYGPSDS 

        70         80         90        100        110        120 
WARFKRMQGF NVFQPMGFDS FGLPAENYAI KTGVHPKDST MKNIETMTKQ LKSMGAMFHW 

       130        140        150        160        170        180 
DNEVITSEPE YYKWTQWMFL QLYKNNLAYR KNAPVNWCPS CNTVLANEQV LDGACERCSS 

       190        200        210        220        230        240 
DVIKKDLTQW FFKITDYAEE LLEKLDDLDW PENTKSMQKH WIGKSIGAQL TFKIVDSDLS 

       250        260        270        280        290        300 
FDIFTTRADT LFGVTYAVLA PENPLVDKIT KEDHKAEIEA YKEQAKKQSE IERQSITREK 

       310        320        330        340        350        360 
TGVFTGSYAI NPINGKKVPV WVGDYVLSTY GTGAVMAVPA HDERDFEFAK KHNLPIEKVI 

       370        380        390        400        410        420 
EGGETLPYTE DGIMINSEEF NGLESSKGRS AVVEKLEKEN LGVKKINYRL RDWLVSRQRY 

       430        440        450        460        470        480 
WGAPIPIVYC DKCGTVAVPE EQLPVKLPYD VEFTPDGKSP LSKCDSFVNT TCPTCGGPAK 

       490        500        510        520        530        540 
REVDTLDTFV CSSWYFLRYA DNKNSEKAFD PKIINEILPV DKYVGGPEHA CMHLLYARFF 

       550        560        570        580        590        600 
TKALRDMGYL NFDEPFSSLT HQGLILGPDG LKMSKSKGNT ISPDDYIDEF GSDVFRMYLM 

       610        620        630        640        650        660 
FGFDYTEGGA WSDEGIKSVS RFVDRVERTL ASCRYYINNP SDDKITIDNN EKDLNFVRHN 

       670        680        690        700        710        720 
SIKSITEDAE KMQFNTCIAR LMEYTNALSK YINEDNKNSK FLKECVEDFI ILIAPFAPHF 

       730        740        750        760        770        780 
SEEQWELLGM TYSVFNEKWP QFDSKALVKD EIEIAVQVNG KIRDRITIAS GLDEESIKET 

       790        800        810 
ALNSEDVKKY TDGKNIVKII IIKGRLVNIV VK 

« Hide

References

[1]"The genome sequence of Clostridium tetani, the causative agent of tetanus disease."
Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Massachusetts / E88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015927 Genomic DNA. Translation: AAO34977.1. Different initiation.
RefSeqNP_781040.1. NC_004557.1.

3D structure databases

ProteinModelPortalQ898V2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING212717.CTC00337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO34977; AAO34977; CTC_00337.
GeneID1059914.
KEGGctc:CTC00337.
PATRIC19508494. VBICloTet101274_0304.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCTET212717:GJAM-296-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CLOTE
AccessionPrimary (citable) accession number: Q898V2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries