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Protein

Putative K(+)-stimulated pyrophosphate-energized sodium pump

Gene

hppA

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Requires K+ for maximal activity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei169 – 1691Important for ion transportBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Metal bindingi180 – 1801Magnesium 1By similarity
Metal bindingi184 – 1841Magnesium 1By similarity
Metal bindingi207 – 2071Magnesium 2By similarity
Metal bindingi210 – 2101Magnesium 2By similarity
Sitei214 – 2141Important for ion transportBy similarity
Sitei221 – 2211Important for ion transportBy similarity
Metal bindingi419 – 4191Magnesium 2By similarity
Sitei449 – 4491Important for potassium dependenceBy similarity
Metal bindingi603 – 6031Calcium or magnesiumBy similarity
Metal bindingi629 – 6291Calcium or magnesiumBy similarity
Metal bindingi633 – 6331Calcium or magnesiumBy similarity
Binding sitei636 – 6361SubstrateBy similarity
Sitei637 – 6371Important for ion transportBy similarity
Sitei648 – 6481Important for ion transportBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Potassium, Sodium

Enzyme and pathway databases

BioCyciCTET212717:GJAM-340-MONOMER.

Protein family/group databases

TCDBi3.A.10.1.7. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative K(+)-stimulated pyrophosphate-energized sodium pumpUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
Na(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:CTC_00383
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001412 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalUniRule annotationAdd
BLAST
Transmembranei62 – 8221HelicalUniRule annotationAdd
BLAST
Transmembranei84 – 10421HelicalUniRule annotationAdd
BLAST
Transmembranei127 – 14721HelicalUniRule annotationAdd
BLAST
Transmembranei154 – 17421HelicalUniRule annotationAdd
BLAST
Transmembranei222 – 24221HelicalUniRule annotationAdd
BLAST
Transmembranei247 – 26721HelicalUniRule annotationAdd
BLAST
Transmembranei279 – 29921HelicalUniRule annotationAdd
BLAST
Transmembranei302 – 32221HelicalUniRule annotationAdd
BLAST
Transmembranei364 – 38421HelicalUniRule annotationAdd
BLAST
Transmembranei387 – 40721HelicalUniRule annotationAdd
BLAST
Transmembranei449 – 46921HelicalUniRule annotationAdd
BLAST
Transmembranei486 – 50621HelicalUniRule annotationAdd
BLAST
Transmembranei553 – 57321HelicalUniRule annotationAdd
BLAST
Transmembranei576 – 59621HelicalUniRule annotationAdd
BLAST
Transmembranei652 – 67221HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 673673Putative K(+)-stimulated pyrophosphate-energized sodium pumpPRO_0000217002Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC00383.

Structurei

3D structure databases

ProteinModelPortaliQ898Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
KOiK15987.
OMAiNVMPALY.
OrthoDBiPOG091H0DQP.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q898Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFIVYSVL AGVIALIFAF MLSSFISKES AGNERMQEIA GHIHDGAMAF
60 70 80 90 100
LKTEYKYLTG FIVIVTVILA IFVGWQTAAC FILGAIFSIF AGYFGMNVAT
110 120 130 140 150
KANVRTAEAA RHSQGKALNI AFSGGAVMGM SVVGLGVVGI GIMYYIFGGN
160 170 180 190 200
MEFVTGFGLG ASSIALFARV GGGIYTKAAD VGADLVGKVE AGIPEDDPRN
210 220 230 240 250
PAVIADNVGD NVGDVAGMGA DLFESYVGSI ISALTLGTVV YANKEGVMFP
260 270 280 290 300
LILSSIGIVA SIIGILFSRK SKAKDPQKAL NTGTYIGGII VIVSAAILSN
310 320 330 340 350
TIFGNLKAFF AVASGLVVGM IIGKITEMYT SDAYSSVQKI ANQSETGPAT
360 370 380 390 400
TIISGLAVGM YSTLWPIVLI SIGVLVSFFV MGGGSNAMVG LYGISLAAVG
410 420 430 440 450
MLSTTGLTVA VDAYGPIADN AGGIAEMSEL PHEVREITDK LDSVGNTTAA
460 470 480 490 500
IGKGFAIGSA ALTALSLFAS YAQATELESI DILNTVTLVG LFIGAMLPFL
510 520 530 540 550
FGALTMESVG KAANEMIEEV RRQFKTIPGI MEGKATPDYK KCVDISTAAA
560 570 580 590 600
IREMILPGVL AIVVPVAMGL LLGKEALGGL LAGALVSGVL VGILMSNAGG
610 620 630 640 650
AWDNAKKYIE GGAHGGKGSE AHKAAVVGDT VGDPFKDTSG PSMNILIKLM
660 670
TIVSLVFAPV VLQYGGILLN LIK
Length:673
Mass (Da):69,282
Last modified:June 1, 2003 - v1
Checksum:iE3505682EE10FF56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA. Translation: AAO35020.1.
RefSeqiWP_011098691.1. NC_004557.1.

Genome annotation databases

EnsemblBacteriaiAAO35020; AAO35020; CTC_00383.
GeneIDi24253865.
KEGGictc:CTC_00383.
PATRICi19508592. VBICloTet101274_0352.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA. Translation: AAO35020.1.
RefSeqiWP_011098691.1. NC_004557.1.

3D structure databases

ProteinModelPortaliQ898Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi212717.CTC00383.

Protein family/group databases

TCDBi3.A.10.1.7. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO35020; AAO35020; CTC_00383.
GeneIDi24253865.
KEGGictc:CTC_00383.
PATRICi19508592. VBICloTet101274_0352.

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
KOiK15987.
OMAiNVMPALY.
OrthoDBiPOG091H0DQP.

Enzyme and pathway databases

BioCyciCTET212717:GJAM-340-MONOMER.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPPA_CLOTE
AccessioniPrimary (citable) accession number: Q898Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.