Reviewed, UniProtKB/Swiss-Prot Q898Q9 (HPPA_CLOTE)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	CTC_00383

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. Potassium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 1 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 673	673	Pyrophosphate-energized proton pump HAMAP MF_01129		PRO_0000217002
Regions
	Transmembrane	4 – 26	23	Potential
	Transmembrane	57 – 74	18	Potential
	Transmembrane	78 – 100	23	Potential
	Transmembrane	121 – 143	23	Potential
	Transmembrane	153 – 175	23	Potential
	Transmembrane	245 – 267	23	Potential
	Transmembrane	282 – 304	23	Potential
	Transmembrane	309 – 331	23	Potential
	Transmembrane	360 – 382	23	Potential
	Transmembrane	389 – 411	23	Potential
	Transmembrane	447 – 469	23	Potential
	Transmembrane	482 – 504	23	Potential
	Transmembrane	554 – 573	20	Potential
	Transmembrane	580 – 602	23	Potential
	Transmembrane	650 – 672	23	Potential
Sites
	Site	449	1	Determinant of potassium dependence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q898Q9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E3505682EE10FF56	FASTA	673	69,282
	10 20 30 40 50 60 MESFIVYSVL AGVIALIFAF MLSSFISKES AGNERMQEIA GHIHDGAMAF LKTEYKYLTG 70 80 90 100 110 120 FIVIVTVILA IFVGWQTAAC FILGAIFSIF AGYFGMNVAT KANVRTAEAA RHSQGKALNI 130 140 150 160 170 180 AFSGGAVMGM SVVGLGVVGI GIMYYIFGGN MEFVTGFGLG ASSIALFARV GGGIYTKAAD 190 200 210 220 230 240 VGADLVGKVE AGIPEDDPRN PAVIADNVGD NVGDVAGMGA DLFESYVGSI ISALTLGTVV 250 260 270 280 290 300 YANKEGVMFP LILSSIGIVA SIIGILFSRK SKAKDPQKAL NTGTYIGGII VIVSAAILSN 310 320 330 340 350 360 TIFGNLKAFF AVASGLVVGM IIGKITEMYT SDAYSSVQKI ANQSETGPAT TIISGLAVGM 370 380 390 400 410 420 YSTLWPIVLI SIGVLVSFFV MGGGSNAMVG LYGISLAAVG MLSTTGLTVA VDAYGPIADN 430 440 450 460 470 480 AGGIAEMSEL PHEVREITDK LDSVGNTTAA IGKGFAIGSA ALTALSLFAS YAQATELESI 490 500 510 520 530 540 DILNTVTLVG LFIGAMLPFL FGALTMESVG KAANEMIEEV RRQFKTIPGI MEGKATPDYK 550 560 570 580 590 600 KCVDISTAAA IREMILPGVL AIVVPVAMGL LLGKEALGGL LAGALVSGVL VGILMSNAGG 610 620 630 640 650 660 AWDNAKKYIE GGAHGGKGSE AHKAAVVGDT VGDPFKDTSG PSMNILIKLM TIVSLVFAPV 670 VLQYGGILLN LIK

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO35020.1.
RefSeq	NP_781083.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1059962.
GenomeReviews	Gene locus CTC_00383 in contig AE015927_GR.
KEGG	ctc:CTC00383.
NMPDR	fig|212717.1.peg.305.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q898Q9.
OMA	PYLFGGI.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_00383-MON.
BRENDA	3.6.1.1. 2082.
Family and domain databases
HAMAP	MF_01129. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_CLOTE

Accession

Primary (citable) accession number: Q898Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)