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Protein

Putative K(+)-stimulated pyrophosphate-energized sodium pump

Gene

hppA

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Requires K+ for maximal activity.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei169Important for ion transportBy similarity1
Binding sitei177SubstrateBy similarity1
Metal bindingi180Magnesium 1By similarity1
Metal bindingi184Magnesium 1By similarity1
Metal bindingi207Magnesium 2By similarity1
Metal bindingi210Magnesium 2By similarity1
Sitei214Important for ion transportBy similarity1
Sitei221Important for ion transportBy similarity1
Metal bindingi419Magnesium 2By similarity1
Sitei449Important for potassium dependenceBy similarity1
Metal bindingi603Calcium or magnesiumBy similarity1
Metal bindingi629Calcium or magnesiumBy similarity1
Metal bindingi633Calcium or magnesiumBy similarity1
Binding sitei636SubstrateBy similarity1
Sitei637Important for ion transportBy similarity1
Sitei648Important for ion transportBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Potassium, Sodium

Protein family/group databases

TCDBi3.A.10.1.7. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative K(+)-stimulated pyrophosphate-energized sodium pumpUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
Na(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:CTC_00383
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001412 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalUniRule annotationAdd BLAST21
Transmembranei62 – 82HelicalUniRule annotationAdd BLAST21
Transmembranei84 – 104HelicalUniRule annotationAdd BLAST21
Transmembranei127 – 147HelicalUniRule annotationAdd BLAST21
Transmembranei154 – 174HelicalUniRule annotationAdd BLAST21
Transmembranei222 – 242HelicalUniRule annotationAdd BLAST21
Transmembranei247 – 267HelicalUniRule annotationAdd BLAST21
Transmembranei279 – 299HelicalUniRule annotationAdd BLAST21
Transmembranei302 – 322HelicalUniRule annotationAdd BLAST21
Transmembranei364 – 384HelicalUniRule annotationAdd BLAST21
Transmembranei387 – 407HelicalUniRule annotationAdd BLAST21
Transmembranei449 – 469HelicalUniRule annotationAdd BLAST21
Transmembranei486 – 506HelicalUniRule annotationAdd BLAST21
Transmembranei553 – 573HelicalUniRule annotationAdd BLAST21
Transmembranei576 – 596HelicalUniRule annotationAdd BLAST21
Transmembranei652 – 672HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002170021 – 673Putative K(+)-stimulated pyrophosphate-energized sodium pumpAdd BLAST673

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC00383.

Structurei

3D structure databases

ProteinModelPortaliQ898Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
KOiK15987.
OMAiNVMPALY.
OrthoDBiPOG091H0DQP.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q898Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFIVYSVL AGVIALIFAF MLSSFISKES AGNERMQEIA GHIHDGAMAF
60 70 80 90 100
LKTEYKYLTG FIVIVTVILA IFVGWQTAAC FILGAIFSIF AGYFGMNVAT
110 120 130 140 150
KANVRTAEAA RHSQGKALNI AFSGGAVMGM SVVGLGVVGI GIMYYIFGGN
160 170 180 190 200
MEFVTGFGLG ASSIALFARV GGGIYTKAAD VGADLVGKVE AGIPEDDPRN
210 220 230 240 250
PAVIADNVGD NVGDVAGMGA DLFESYVGSI ISALTLGTVV YANKEGVMFP
260 270 280 290 300
LILSSIGIVA SIIGILFSRK SKAKDPQKAL NTGTYIGGII VIVSAAILSN
310 320 330 340 350
TIFGNLKAFF AVASGLVVGM IIGKITEMYT SDAYSSVQKI ANQSETGPAT
360 370 380 390 400
TIISGLAVGM YSTLWPIVLI SIGVLVSFFV MGGGSNAMVG LYGISLAAVG
410 420 430 440 450
MLSTTGLTVA VDAYGPIADN AGGIAEMSEL PHEVREITDK LDSVGNTTAA
460 470 480 490 500
IGKGFAIGSA ALTALSLFAS YAQATELESI DILNTVTLVG LFIGAMLPFL
510 520 530 540 550
FGALTMESVG KAANEMIEEV RRQFKTIPGI MEGKATPDYK KCVDISTAAA
560 570 580 590 600
IREMILPGVL AIVVPVAMGL LLGKEALGGL LAGALVSGVL VGILMSNAGG
610 620 630 640 650
AWDNAKKYIE GGAHGGKGSE AHKAAVVGDT VGDPFKDTSG PSMNILIKLM
660 670
TIVSLVFAPV VLQYGGILLN LIK
Length:673
Mass (Da):69,282
Last modified:June 1, 2003 - v1
Checksum:iE3505682EE10FF56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA. Translation: AAO35020.1.
RefSeqiWP_011098691.1. NC_004557.1.

Genome annotation databases

EnsemblBacteriaiAAO35020; AAO35020; CTC_00383.
GeneIDi24253865.
KEGGictc:CTC_00383.
PATRICi19508592. VBICloTet101274_0352.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA. Translation: AAO35020.1.
RefSeqiWP_011098691.1. NC_004557.1.

3D structure databases

ProteinModelPortaliQ898Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi212717.CTC00383.

Protein family/group databases

TCDBi3.A.10.1.7. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO35020; AAO35020; CTC_00383.
GeneIDi24253865.
KEGGictc:CTC_00383.
PATRICi19508592. VBICloTet101274_0352.

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
KOiK15987.
OMAiNVMPALY.
OrthoDBiPOG091H0DQP.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPPA_CLOTE
AccessioniPrimary (citable) accession number: Q898Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.