Q898E6 (IOLE_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inosose dehydratase
EC=4.2.1.44

Alternative name(s):
2-keto-myo-inositol dehydratase
Short name=2KMI dehydratase

Gene names

Name:	iolE
Ordered Locus Names:	CTC_00512

Organism

Clostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]

Taxonomic identifier

212717 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium tetani

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dehydration of inosose (2-keto-myo-inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol) By similarity. HAMAP-Rule MF_01672
Catalytic activity	2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O. HAMAP-Rule MF_01672
Cofactor	Glutathione By similarity. Cobalt or manganese By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 2/7. HAMAP-Rule MF_01672
Sequence similarities	Belongs to the IolE/MocC family.

Ontologies

Keywords
Ligand	Cobalt Manganese
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	manganese ion binding Inferred from electronic annotation. Source: HAMAP myo-inosose-2 dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Inosose dehydratase HAMAP-Rule MF_01672		PRO_0000352366

Sequences

	Sequence		Length	Mass (Da)
	Q898E6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7D302076FED20284	FASTA	298	34,217
	10 20 30 40 50 60 MFNKDKVKIG IAPIAWTNDD MPELGAENTF EQCISEMALS GFKGTEVGNK YPRDTKVLKR 70 80 90 100 110 120 ALELRDMQIA SAWFSAFLTT KPYEETEKAF IKHRDFLYEM RSKVIVISEQ GHSIQGKMET 130 140 150 160 170 180 PIFEEKPIFT EEEWKDLAKG LNKLGKLAKE KNMKVVYHHH MGTGVQTTEE IDKLMEMTDP 190 200 210 220 230 240 TLVYLLYDTG HLVFSGEDPI VVLRKYINRI KHVHLKDIRE DVVKIVKQKK MSFLQAVKLG 250 260 270 280 290 AFTVPGDGDI DFKPVFNILA ENNYEGWLLV EAEQDPARAN PLEYAIKARK YIQEKAAI

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO35135.1.
RefSeq	NP_781198.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q898E6.
ModBase	Search...
Protein-protein interaction databases
STRING	212717.CTC00512.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO35135; AAO35135; CTC_00512.
GeneID	1060116.
KEGG	ctc:CTC00512.
PATRIC	19508848. VBICloTet101274_0480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1082.
KO	K03335.
OMA	DMPDLGK.
ProtClustDB	CLSK810783.
Enzyme and pathway databases
BioCyc	CTET212717:GJAM-455-MONOMER.
UniPathway	UPA00076; UER00144.
Family and domain databases
Gene3D	3.20.20.150. 1 hit.
HAMAP	MF_01672. IolE.
InterPro	IPR023952. IolE. IPR013022. Xyl_isomerase-like_TIM-brl. [Graphical view]
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
SUPFAM	SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IOLE_CLOTE

Accession

Primary (citable) accession number: Q898E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program