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Q89822 (REPA_SSVN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication-associated protein A

Short name=RepA
EC=3.1.21.-
Gene names
ORF Names:C1
OrganismSugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal)) [Complete proteome]
Taxonomic identifier268781 [NCBI]
Taxonomic lineageVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostCenchrus echinatus [TaxID: 173841]
Saccharum officinarum (Sugarcane) [TaxID: 4547]

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription By similarity.

Subunit structure

Homooligomer. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription By similarity.

Subcellular location

Host nucleus By similarity. Host cytoplasm By similarity.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

The LXCXE motif specifically binds to host RBR1 By similarity.

Sequence similarities

Belongs to the geminiviridae Rep protein family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform RepA (identifier: Q89822-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced from the unspliced transcript.
Isoform Rep (identifier: Q80GM6-1)

The sequence of this isoform can be found in the external entry Q80GM6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Replication-associated protein A
PRO_0000316944

Regions

Region180 – 19213Oligomerization By similarity
Motif24 – 285RCR-1
Motif66 – 716RCR-2
Motif106 – 1094RCR-3

Sites

Active site1061For DNA cleavage activity By similarity
Metal binding581Divalent metal cation Potential
Metal binding661Divalent metal cation Potential
Metal binding681Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform RepA [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 33854BB4235234F3

FASTA31434,940
        10         20         30         40         50         60 
MSTVGSTVSS TPSRRFKHRN VNTFLTYSRC PLEPEAVGLH IWSLIAHWTP VYVLSVRETH 

        70         80         90        100        110        120 
EDGGYHIHVL AQSAKPVYTT DSGFFDIDGF HPNIQSAKSA NKVRAYAMKN PVTYWERGTF 

       130        140        150        160        170        180 
IPRKTSFLGD STEPNSKKQS KDDIVRDIIE HSTNKQEYLS MIQKALPYEW ATKLQYFEYS 

       190        200        210        220        230        240 
ANKLFPDIQE IYTSPFPQST PALLDPTAIN TWLENNLYQV SPSAYMLANP SCLTLEEATS 

       250        260        270        280        290        300 
DLIWMHETSR TLIPTGSSAC TSSGQQEQAS PPGPGAWEDI ITGRTTSTGP PTTRTQNTTS 

       310 
STTYPSSTVH AGSN 

« Hide

Isoform Rep [UniParc].

See Q80GM6.

References

[1]"Molecular investigations of subgroup 1 geminiviruses."
Hughes F.L.
Thesis (1991), University of Cape Town, South Africa
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of sugarcane streak Monogeminivirus."
Hughes F.L., Rybicki E.P., Kirby R.
Arch. Virol. 132:171-182(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M82918 Genomic DNA. Translation: AAA47830.1.
S64567 Genomic DNA. Translation: AAP13957.1.

3D structure databases

ProteinModelPortalQ89822.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.
ProtoNetSearch...

Entry information

Entry nameREPA_SSVN
AccessionPrimary (citable) accession number: Q89822
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families