Q89822 (REPA_SSVN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication-associated protein A
Short name=RepA
EC=3.1.21.-

Gene names

ORF Names:

C1

Organism

Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal)) [Complete proteome]

Taxonomic identifier

268781 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Geminiviridae › Mastrevirus › Sugarcane streak virus

Virus host

Cenchrus echinatus [TaxID: 173841]
Saccharum officinarum (Sugarcane) [TaxID: 4547]

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription By similarity.
Subunit structure	Homooligomer. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription By similarity.
Subcellular location	Host nucleus By similarity. Host cytoplasm By similarity.
Domain	There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR. The LXCXE motif specifically binds to host RBR1 By similarity.
Sequence similarities	Belongs to the geminiviridae Rep protein family.

Ontologies

Keywords
Biological process	G1/S host cell cycle checkpoint dysregulation by virus Host-virus interaction Modulation of host cell cycle by virus
Cellular component	Host cytoplasm Host nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding Metal-binding
Molecular function	Activator Endonuclease Hydrolase Nuclease Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: InterPro modulation by virus of host G1/S transition checkpoint Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endodeoxyribonuclease activity, producing 5'-phosphomonoesters Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform RepA (identifier: Q89822-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced from the unspliced transcript.

Isoform Rep (identifier: Q80GM6-1)

The sequence of this isoform can be found in the external entry Q80GM6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Replication-associated protein A		PRO_0000316944
Regions
	Region	180 – 192	13	Oligomerization By similarity
	Motif	24 – 28	5	RCR-1
	Motif	66 – 71	6	RCR-2
	Motif	106 – 109	4	RCR-3
Sites
	Active site	106	1	For DNA cleavage activity By similarity
	Metal binding	58	1	Divalent metal cation Potential
	Metal binding	66	1	Divalent metal cation Potential
	Metal binding	68	1	Divalent metal cation Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform RepA [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 33854BB4235234F3	FASTA	314	34,940
	10 20 30 40 50 60 MSTVGSTVSS TPSRRFKHRN VNTFLTYSRC PLEPEAVGLH IWSLIAHWTP VYVLSVRETH 70 80 90 100 110 120 EDGGYHIHVL AQSAKPVYTT DSGFFDIDGF HPNIQSAKSA NKVRAYAMKN PVTYWERGTF 130 140 150 160 170 180 IPRKTSFLGD STEPNSKKQS KDDIVRDIIE HSTNKQEYLS MIQKALPYEW ATKLQYFEYS 190 200 210 220 230 240 ANKLFPDIQE IYTSPFPQST PALLDPTAIN TWLENNLYQV SPSAYMLANP SCLTLEEATS 250 260 270 280 290 300 DLIWMHETSR TLIPTGSSAC TSSGQQEQAS PPGPGAWEDI ITGRTTSTGP PTTRTQNTTS 310 STTYPSSTVH AGSN
	Isoform Rep [UniParc]. See Q80GM6.		–	–

References

[1]	"Molecular investigations of subgroup 1 geminiviruses." Hughes F.L. Thesis (1991), University of Cape Town, South Africa Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of sugarcane streak Monogeminivirus." Hughes F.L., Rybicki E.P., Kirby R. Arch. Virol. 132:171-182(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M82918 Genomic DNA. Translation: AAA47830.1. S64567 Genomic DNA. Translation: AAP13957.1.
3D structure databases
HSSP	HSSP built from PDB template 1L5I based on UniProtKB P27260.
ProteinModelPortal	Q89822.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001146. Gemini_AL1_MSV. IPR001191. Gemini_AL1_REP. IPR022690. Gemini_AL1_REP_cat-dom. IPR022692. Gemini_AL1_REP_central. [Graphical view]
Pfam	PF00799. Gemini_AL1. 1 hit. PF08283. Gemini_AL1_M. 1 hit. [Graphical view]
PRINTS	PR00227. GEMCOATAL1. PR00229. GEMCOATMSVL1.
ProtoNet	Search...

Entry information

Entry name

REPA_SSVN

Accession

Primary (citable) accession number: Q89822

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program