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Q89822

- REPA_SSVN

UniProt

Q89822 - REPA_SSVN

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Protein

Replication-associated protein A

Gene

C1

Organism
Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Divalent metal cationSequence Analysis
Metal bindingi66 – 661Divalent metal cationSequence Analysis
Metal bindingi68 – 681Divalent metal cationSequence Analysis
Active sitei106 – 1061For DNA cleavage activityBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. structural molecule activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: InterPro
  2. modulation by virus of host G1/S transition checkpoint Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Endonuclease, Hydrolase, Nuclease, Repressor

Keywords - Biological processi

G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus

Keywords - Ligandi

DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein A (EC:3.1.21.-)
Short name:
RepA
Gene namesi
ORF Names:C1
OrganismiSugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal))
Taxonomic identifieri268781 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostiCenchrus echinatus [TaxID: 173841]
Saccharum officinarum (Sugarcane) [TaxID: 4547]
ProteomesiUP000007230: Genome

Subcellular locationi

Host nucleus By similarity. Host cytoplasm By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral capsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Replication-associated protein APRO_0000316944Add
BLAST

Interactioni

Subunit structurei

Homooligomer. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ89822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 19213OligomerizationBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi24 – 285RCR-1
Motifi66 – 716RCR-2
Motifi106 – 1094RCR-3

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.
The LXCXE motif specifically binds to host RBR1.By similarity

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.Curated

Family and domain databases

InterProiIPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view]
PfamiPF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view]
PRINTSiPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform RepA (identifier: Q89822-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTVGSTVSS TPSRRFKHRN VNTFLTYSRC PLEPEAVGLH IWSLIAHWTP
60 70 80 90 100
VYVLSVRETH EDGGYHIHVL AQSAKPVYTT DSGFFDIDGF HPNIQSAKSA
110 120 130 140 150
NKVRAYAMKN PVTYWERGTF IPRKTSFLGD STEPNSKKQS KDDIVRDIIE
160 170 180 190 200
HSTNKQEYLS MIQKALPYEW ATKLQYFEYS ANKLFPDIQE IYTSPFPQST
210 220 230 240 250
PALLDPTAIN TWLENNLYQV SPSAYMLANP SCLTLEEATS DLIWMHETSR
260 270 280 290 300
TLIPTGSSAC TSSGQQEQAS PPGPGAWEDI ITGRTTSTGP PTTRTQNTTS
310
STTYPSSTVH AGSN

Note: Produced from the unspliced transcript.

Length:314
Mass (Da):34,940
Last modified:November 1, 1996 - v1
Checksum:i33854BB4235234F3
GO
Isoform Rep (identifier: Q80GM6-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q80GM6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:364
Mass (Da):41,819
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82918 Genomic DNA. Translation: AAA47830.1.
S64567 Genomic DNA. Translation: AAP13957.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82918 Genomic DNA. Translation: AAA47830.1 .
S64567 Genomic DNA. Translation: AAP13957.1 .

3D structure databases

ProteinModelPortali Q89822.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
[Graphical view ]
Pfami PF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
[Graphical view ]
PRINTSi PR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.
ProtoNeti Search...

Publicationsi

  1. "Molecular investigations of subgroup 1 geminiviruses."
    Hughes F.L.
    Thesis (1991), University of Cape Town, South Africa
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of sugarcane streak Monogeminivirus."
    Hughes F.L., Rybicki E.P., Kirby R.
    Arch. Virol. 132:171-182(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiREPA_SSVN
AccessioniPrimary (citable) accession number: Q89822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3