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Q89822

- REPA_SSVN

UniProt

Q89822 - REPA_SSVN

Protein

Replication-associated protein A

Gene

C1

Organism
Sugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi58 – 581Divalent metal cationSequence Analysis
    Metal bindingi66 – 661Divalent metal cationSequence Analysis
    Metal bindingi68 – 681Divalent metal cationSequence Analysis
    Active sitei106 – 1061For DNA cleavage activityBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. structural molecule activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: InterPro
    2. modulation by virus of host G1/S transition checkpoint Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Endonuclease, Hydrolase, Nuclease, Repressor

    Keywords - Biological processi

    G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus

    Keywords - Ligandi

    DNA-binding, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication-associated protein A (EC:3.1.21.-)
    Short name:
    RepA
    Gene namesi
    ORF Names:C1
    OrganismiSugarcane streak virus (isolate South Africa) (SSV) (Sugarcane streak virus (isolate Natal))
    Taxonomic identifieri268781 [NCBI]
    Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
    Virus hostiCenchrus echinatus [TaxID: 173841]
    Saccharum officinarum (Sugarcane) [TaxID: 4547]
    ProteomesiUP000007230: Genome

    Subcellular locationi

    Host nucleus By similarity. Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral capsid Source: InterPro

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314Replication-associated protein APRO_0000316944Add
    BLAST

    Interactioni

    Subunit structurei

    Homooligomer. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ89822.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni180 – 19213OligomerizationBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi24 – 285RCR-1
    Motifi66 – 716RCR-2
    Motifi106 – 1094RCR-3

    Domaini

    There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.
    The LXCXE motif specifically binds to host RBR1.By similarity

    Sequence similaritiesi

    Belongs to the geminiviridae Rep protein family.Curated

    Family and domain databases

    InterProiIPR001146. Gemini_AL1_MSV.
    IPR001191. Gemini_AL1_REP.
    IPR022690. Gemini_AL1_REP_cat-dom.
    IPR022692. Gemini_AL1_REP_central.
    [Graphical view]
    PfamiPF00799. Gemini_AL1. 1 hit.
    PF08283. Gemini_AL1_M. 1 hit.
    [Graphical view]
    PRINTSiPR00227. GEMCOATAL1.
    PR00229. GEMCOATMSVL1.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform RepA (identifier: Q89822-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSTVGSTVSS TPSRRFKHRN VNTFLTYSRC PLEPEAVGLH IWSLIAHWTP    50
    VYVLSVRETH EDGGYHIHVL AQSAKPVYTT DSGFFDIDGF HPNIQSAKSA 100
    NKVRAYAMKN PVTYWERGTF IPRKTSFLGD STEPNSKKQS KDDIVRDIIE 150
    HSTNKQEYLS MIQKALPYEW ATKLQYFEYS ANKLFPDIQE IYTSPFPQST 200
    PALLDPTAIN TWLENNLYQV SPSAYMLANP SCLTLEEATS DLIWMHETSR 250
    TLIPTGSSAC TSSGQQEQAS PPGPGAWEDI ITGRTTSTGP PTTRTQNTTS 300
    STTYPSSTVH AGSN 314

    Note: Produced from the unspliced transcript.

    Length:314
    Mass (Da):34,940
    Last modified:November 1, 1996 - v1
    Checksum:i33854BB4235234F3
    GO
    Isoform Rep (identifier: Q80GM6-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q80GM6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:364
    Mass (Da):41,819
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82918 Genomic DNA. Translation: AAA47830.1.
    S64567 Genomic DNA. Translation: AAP13957.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82918 Genomic DNA. Translation: AAA47830.1 .
    S64567 Genomic DNA. Translation: AAP13957.1 .

    3D structure databases

    ProteinModelPortali Q89822.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001146. Gemini_AL1_MSV.
    IPR001191. Gemini_AL1_REP.
    IPR022690. Gemini_AL1_REP_cat-dom.
    IPR022692. Gemini_AL1_REP_central.
    [Graphical view ]
    Pfami PF00799. Gemini_AL1. 1 hit.
    PF08283. Gemini_AL1_M. 1 hit.
    [Graphical view ]
    PRINTSi PR00227. GEMCOATAL1.
    PR00229. GEMCOATMSVL1.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular investigations of subgroup 1 geminiviruses."
      Hughes F.L.
      Thesis (1991), University of Cape Town, South Africa
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete nucleotide sequence of sugarcane streak Monogeminivirus."
      Hughes F.L., Rybicki E.P., Kirby R.
      Arch. Virol. 132:171-182(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiREPA_SSVN
    AccessioniPrimary (citable) accession number: Q89822
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3