Reviewed,
UniProtKB/Swiss-Prot Q897P8 (DDLA_CLOTE)
Last modified
November 25, 2008.
Version 43.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: D-alanine--D-alanine ligase A EC=6.3.2.4 Alternative name(s): D-alanylalanine synthetase A D-Ala-D-Ala ligase A | ||||
| Gene names |
| ||||
| Organism | Clostridium tetani [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 1513 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium |
Protein attributes
| Sequence length | 301 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Cell wall formation By similarity. |
| Catalytic activity | ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. |
| Cofactor | Binds 2 magnesium or manganese ions per subunit By similarity. |
| Pathway | |
| Subcellular location | CytoplasmBy similarity. |
| Sequence similarities | Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shapeInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cell wall Inferred from electronic annotation. Source: InterPro cytoplasmInferred from electronic annotation. Source: HAMAP |
| Molecular function | ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activityInferred from electronic annotation. Source: HAMAP magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 301 | 301 | D-alanine--D-alanine ligase A | PRO_0000177809 | |||||
Regions | |||||||||
| Domain | 99 – 293 | 195 | ATP-grasp | ||||||
| Nucleotide binding | 126 – 181 | 56 | ATP By similarity | ||||||
Sites | |||||||||
| Metal binding | 248 | 1 | Magnesium or manganese 1 By similarity | ||||||
| Metal binding | 260 | 1 | Magnesium or manganese 1 By similarity | ||||||
| Metal binding | 260 | 1 | Magnesium or manganese 2 By similarity | ||||||
| Metal binding | 262 | 1 | Magnesium or manganese 2 By similarity | ||||||
Sequences
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References
| [1] | "The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88. |
Cross-references
Sequence databases | |
|---|---|
| AE015927 Genomic DNA. Translation: AAO35288.1. | |
| RefSeq | NP_781351.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IOW based on UniProtKB P07862. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1058953. |
| GenomeReviews | Gene locus CTC_00683 in contig AE015927_GR. |
| KEGG | ctc:CTC00683. |
| NMPDR | fig|212717.1.peg.573. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q897P8. |
Enzyme and pathway databases | |
| BioCyc | CTET212717:CTC_00683-MON. |
Family and domain databases | |
| HAMAP | MF_00047. [Tree] |
| InterPro | IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view] |
| Gene3D | G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01205. D_ala_D_alaTIGR. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DDLA_CLOTE | ||||||||
| Accession | Primary (citable) accession number: Q897P8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


