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Q897P8 (DDLA_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase A

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetase A
Gene names
Name:ddlA
Ordered Locus Names:CTC_00683
OrganismClostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]
Taxonomic identifier212717 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301D-alanine--D-alanine ligase A HAMAP-Rule MF_00047
PRO_0000177809

Regions

Domain99 – 293195ATP-grasp
Nucleotide binding126 – 18156ATP By similarity

Sites

Metal binding2481Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 2 By similarity
Metal binding2621Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q897P8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: AEC30BCA0618FDF5

FASTA30134,031
        10         20         30         40         50         60 
MQIGVVMGGV STEKEISNLT GKYIIENLDK SKYEILPIPI NTKFELIDKI KCLEFAFIAL 

        70         80         90        100        110        120 
HGNFGEDGKV QALLETMGVP YSGSGVLASS LCMDKNMSKK ILQGEGIKTP RWIMLYKNED 

       130        140        150        160        170        180 
IDFESIKNIG YPLVVKPNSG GSSIGITIVK KEQELIKAIE EAFKFDEEIL IEEYIKGEEI 

       190        200        210        220        230        240 
TCCMLDGKPL PILSIKTKEE FFNYKAKYFE GVAEEKVADL SLELKDQVEK ISNKCWKSFK 

       250        260        270        280        290        300 
LKVYGRIDMI IKGNEVYVIE INTLPGMTKY SLFPKSAKAY GLNFSELLDK IIELSIKEYE 


F 

« Hide

References

[1]"The genome sequence of Clostridium tetani, the causative agent of tetanus disease."
Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G.
Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Massachusetts / E88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015927 Genomic DNA. Translation: AAO35288.1.
RefSeqNP_781351.1. NC_004557.1.

3D structure databases

ProteinModelPortalQ897P8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING212717.CTC00683.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO35288; AAO35288; CTC_00683.
GeneID1058953.
KEGGctc:CTC00683.
PATRIC19509187. VBICloTet101274_0643.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMADRIQAND.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycCTET212717:GJAM-614-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLA_CLOTE
AccessionPrimary (citable) accession number: Q897P8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways