Reviewed, UniProtKB/Swiss-Prot Q897P8 (DDLA_CLOTE)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase A
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase A
    D-Ala-D-Ala ligase A

Gene names

Name:	ddlA
Ordered Locus Names:	CTC_00683

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	D-alanine--D-alanine ligase A		PRO_0000177809
Regions
	Domain	99 – 293	195	ATP-grasp
	Nucleotide binding	126 – 181	56	ATP By similarity
Sites
	Metal binding	248	1	Magnesium or manganese 1 By similarity
	Metal binding	260	1	Magnesium or manganese 1 By similarity
	Metal binding	260	1	Magnesium or manganese 2 By similarity
	Metal binding	262	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q897P8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: AEC30BCA0618FDF5	FASTA	301	34,031
	10 20 30 40 50 60 MQIGVVMGGV STEKEISNLT GKYIIENLDK SKYEILPIPI NTKFELIDKI KCLEFAFIAL 70 80 90 100 110 120 HGNFGEDGKV QALLETMGVP YSGSGVLASS LCMDKNMSKK ILQGEGIKTP RWIMLYKNED 130 140 150 160 170 180 IDFESIKNIG YPLVVKPNSG GSSIGITIVK KEQELIKAIE EAFKFDEEIL IEEYIKGEEI 190 200 210 220 230 240 TCCMLDGKPL PILSIKTKEE FFNYKAKYFE GVAEEKVADL SLELKDQVEK ISNKCWKSFK 250 260 270 280 290 300 LKVYGRIDMI IKGNEVYVIE INTLPGMTKY SLFPKSAKAY GLNFSELLDK IIELSIKEYE F

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO35288.1.
RefSeq	NP_781351.1.
3D structure databases
HSSP	HSSP built from PDB template 1IOW based on UniProtKB P07862.
ModBase	Search...
Genome annotation databases
GeneID	1058953.
GenomeReviews	Gene locus CTC_00683 in contig AE015927_GR.
KEGG	ctc:CTC00683.
NMPDR	fig|212717.1.peg.573.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q897P8.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_00683-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDLA_CLOTE

Accession

Primary (citable) accession number: Q897P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)