Q897P8 (DDLA_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase A
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetase A

Gene names

Name:	ddlA
Ordered Locus Names:	CTC_00683

Organism

Clostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]

Taxonomic identifier

212717 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium tetani

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	D-alanine--D-alanine ligase A HAMAP-Rule MF_00047		PRO_0000177809
Regions
	Domain	99 – 293	195	ATP-grasp
	Nucleotide binding	126 – 181	56	ATP By similarity
Sites
	Metal binding	248	1	Magnesium or manganese 1 By similarity
	Metal binding	260	1	Magnesium or manganese 1 By similarity
	Metal binding	260	1	Magnesium or manganese 2 By similarity
	Metal binding	262	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q897P8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: AEC30BCA0618FDF5	FASTA	301	34,031
	10 20 30 40 50 60 MQIGVVMGGV STEKEISNLT GKYIIENLDK SKYEILPIPI NTKFELIDKI KCLEFAFIAL 70 80 90 100 110 120 HGNFGEDGKV QALLETMGVP YSGSGVLASS LCMDKNMSKK ILQGEGIKTP RWIMLYKNED 130 140 150 160 170 180 IDFESIKNIG YPLVVKPNSG GSSIGITIVK KEQELIKAIE EAFKFDEEIL IEEYIKGEEI 190 200 210 220 230 240 TCCMLDGKPL PILSIKTKEE FFNYKAKYFE GVAEEKVADL SLELKDQVEK ISNKCWKSFK 250 260 270 280 290 300 LKVYGRIDMI IKGNEVYVIE INTLPGMTKY SLFPKSAKAY GLNFSELLDK IIELSIKEYE F

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO35288.1.
RefSeq	NP_781351.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q897P8.
ModBase	Search...
Protein-protein interaction databases
STRING	212717.CTC00683.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO35288; AAO35288; CTC_00683.
GeneID	1058953.
KEGG	ctc:CTC00683.
PATRIC	19509187. VBICloTet101274_0643.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
KO	K01921.
OMA	GNEMIAH.
ProtClustDB	PRK01372.
Enzyme and pathway databases
BioCyc	CTET212717:GJAM-614-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDLA_CLOTE

Accession

Primary (citable) accession number: Q897P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program