Reviewed, UniProtKB/Swiss-Prot Q897F7 (ASNA_CLOTE)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	CTC_00781

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195872

Sequences

	Sequence		Length	Mass (Da)
	Q897F7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 709CB138963B7438	FASTA	341	40,005
	10 20 30 40 50 60 MEIKCSERLI IPKEYKTELD LKETAIAIKE VKDCFERALA KQLNLIRVSA PLFVRCDKGL 70 80 90 100 110 120 NDNLNGVERP VKFTVKDDNE AAVEIVHSLA KWKRMALYRY NFNADEGLYT DMNAIRRDEE 130 140 150 160 170 180 LDNTHSIYVD QWDWERIIKK EDRNEEYLKD IVRKIFKAFK ETEEHINKLY PFLGEVLPEE 190 200 210 220 230 240 VFFMTTQELE DMFPDLTAKE REDAITKEKK AVFLMKIGKT LESGEKHDGR APDYDDWELN 250 260 270 280 290 300 GDILFWNPVL NKAFELSSMG IRVDEESLLK QLKLANCEER KELQFHKMLL EKKLPYTIGG 310 320 330 340 GIGQSRMCML FLKKAHIGEV QSSIWPEEMI KFCEEKGMTI L

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO35379.1.
RefSeq	NP_781442.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
ModBase	Search...
Genome annotation databases
GeneID	1060794.
GenomeReviews	Gene locus CTC_00781 in contig AE015927_GR.
KEGG	ctc:CTC00781.
NMPDR	fig|212717.1.peg.664.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q897F7.
OMA	LNDNLNG.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_00781-MON.
BRENDA	6.3.1.1. 2082.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_CLOTE

Accession

Primary (citable) accession number: Q897F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)