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Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs bound to the protein M1. Since protein M1 is not available in large quantities before late stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the host nucleus until sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enter the host cytoplasm only when associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.UniRule annotation

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export proteinUniRule annotation
Short name:
NEPUniRule annotation
Alternative name(s):
Non-structural protein 2UniRule annotation
Short name:
NS2UniRule annotation
Gene namesi
Name:NSUniRule annotation
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000790141 – 121Nuclear export proteinAdd BLAST121

Interactioni

Subunit structurei

Interacts with protein M1. May interact with host nucleoporin RAB/HRB and exportin XPO1/CRM1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ89733.
SMRiQ89733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 21Nuclear export signalUniRule annotation10
Motifi85 – 94Nuclear export signalUniRule annotation10

Sequence similaritiesi

Belongs to the influenza viruses NEP family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000NT.

Family and domain databases

HAMAPiMF_04067. INFV_NEP. 1 hit.
InterProiView protein in InterPro
IPR000968. Flu_NS2.
PfamiView protein in Pfam
PF00601. Flu_NS2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform NEP (identifier: Q89733-1) [UniParc]FASTAAdd to basket
Also known as: NS2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPNTVSSFQ DILMRMSKMQ LGSSSEDLNG IITQFESLKL YRDSLGEAVM
60 70 80 90 100
RMGDLHSLQN RNGKWREQLG QKFEEIRWLI EEVRHRLKIT ENSFEQITFM
110 120
QALQLLLEVE QEIRTFSFQL I
Length:121
Mass (Da):14,327
Last modified:November 1, 1996 - v1
Checksum:iB15C14C594F55922
GO
Isoform NS1 (identifier: Q82506-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q82506.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:230
Mass (Da):26,006
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13682 mRNA. Translation: AAA21581.1.
U13683 Genomic RNA. Translation: AAA21583.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13682 mRNA. Translation: AAA21581.1.
U13683 Genomic RNA. Translation: AAA21583.1.

3D structure databases

ProteinModelPortaliQ89733.
SMRiQ89733.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090000NT.

Family and domain databases

HAMAPiMF_04067. INFV_NEP. 1 hit.
InterProiView protein in InterPro
IPR000968. Flu_NS2.
PfamiView protein in Pfam
PF00601. Flu_NS2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNEP_I33A0
AccessioniPrimary (citable) accession number: Q89733
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.