Q89733 (NEP_I33A0) Reviewed, UniProtKB/Swiss-Prot
Last modified February 19, 2014. Version 50. History...
Names and origin
|Protein names||Recommended name:|
Nuclear export protein
Non-structural protein 2
|Organism||Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) [Complete proteome]|
|Taxonomic identifier||381518 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A ›|
|Virus host||Aves [TaxID: 8782]|
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
|Sequence length||121 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced By similarity.
Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 By similarity.
Average number present in a viral particle is estimated to be 130-200 molecules.
Belongs to the influenza viruses NEP family.
|Biological process||Host-virus interaction|
|Cellular component||Host nucleus|
|Coding sequence diversity||Alternative splicing|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||RNA export from nucleus|
Inferred from electronic annotation. Source: InterProviral process
Inferred from electronic annotation. Source: UniProtKB-KW
|Cellular_component||host cell nucleus|
Inferred from electronic annotation. Source: UniProtKB-SubCellvirion
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Complete GO annotation...|
|This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]|
|Isoform NEP (identifier: Q89733-1) |
Also known as: NS2;
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform NS1 (identifier: Q82506-1) |
The sequence of this isoform can be found in the external entry Q82506.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence annotation (Features)
Thesis (1994), Robert Wood Johnson Medical School, United States
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|||"Influenza A virus NS2 protein mediates vRNP nuclear export through NES-independent interaction with hCRM1."|
Neumann G., Hughes M.T., Kawaoka Y.
EMBO J. 19:6751-6758(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN XPO1.
|U13682 mRNA. Translation: AAA21581.1.|
U13683 Genomic RNA. Translation: AAA21583.1.
3D structure databases
|SMR||Q89733. Positions 63-116. |
Protocols and materials databases
Family and domain databases
|InterPro||IPR000968. Flu_NS2. |
|Pfam||PF00601. Flu_NS2. 1 hit. |
|Accession||Primary (citable) accession number: Q89733|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families