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Q89733

- NEP_I33A0

UniProt

Q89733 - NEP_I33A0

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Protein

Nuclear export protein

Gene
NS
Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced By similarity.

GO - Biological processi

  1. RNA export from nucleus Source: InterPro
  2. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export protein
Short name:
NEP
Alternative name(s):
Non-structural protein 2
Short name:
NS2
Gene namesi
Name:NS
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Subcellular locationi

Virion Reviewed prediction. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Nuclear export proteinPRO_0000079014Add
BLAST

Interactioni

Subunit structurei

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ89733.
SMRiQ89733. Positions 63-116.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2110Nuclear export signal By similarity
Motifi85 – 9410Nuclear export signal By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR000968. Flu_NS2.
[Graphical view]
PfamiPF00601. Flu_NS2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform NEP (identifier: Q89733-1) [UniParc]FASTAAdd to Basket

Also known as: NS2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDPNTVSSFQ DILMRMSKMQ LGSSSEDLNG IITQFESLKL YRDSLGEAVM    50
RMGDLHSLQN RNGKWREQLG QKFEEIRWLI EEVRHRLKIT ENSFEQITFM 100
QALQLLLEVE QEIRTFSFQL I 121
Length:121
Mass (Da):14,327
Last modified:November 1, 1996 - v1
Checksum:iB15C14C594F55922
GO
Isoform NS1 (identifier: Q82506-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q82506.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:230
Mass (Da):26,006
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13682 mRNA. Translation: AAA21581.1.
U13683 Genomic RNA. Translation: AAA21583.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13682 mRNA. Translation: AAA21581.1 .
U13683 Genomic RNA. Translation: AAA21583.1 .

3D structure databases

ProteinModelPortali Q89733.
SMRi Q89733. Positions 63-116.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000968. Flu_NS2.
[Graphical view ]
Pfami PF00601. Flu_NS2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Husak P.J.
    Thesis (1994), Robert Wood Johnson Medical School, United States
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Influenza A virus NS2 protein mediates vRNP nuclear export through NES-independent interaction with hCRM1."
    Neumann G., Hughes M.T., Kawaoka Y.
    EMBO J. 19:6751-6758(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN XPO1.

Entry informationi

Entry nameiNEP_I33A0
AccessioniPrimary (citable) accession number: Q89733
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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