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Q896M5

- SYE_CLOTE

UniProt

Q896M5 - SYE_CLOTE

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Protein

Glutamate--tRNA ligase

Gene
gltX, CTC_00977
Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Zinc By similarity
Metal bindingi111 – 1111Zinc By similarity
Metal bindingi136 – 1361Zinc By similarity
Metal bindingi138 – 1381Zinc By similarity
Binding sitei256 – 2561ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTET212717:GJAM-893-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:CTC_00977
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001412: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Glutamate--tRNA ligaseUniRule annotationPRO_0000119546Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC00977.

Structurei

3D structure databases

ProteinModelPortaliQ896M5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionUniRule annotationAdd
BLAST
Motifi253 – 2575"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
KOiK01885.
OMAiKLEWYNG.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q896M5-1 [UniParc]FASTAAdd to Basket

« Hide

MNGNKIRTRF APSPTGYMHV GNLRTALYAY LIAKHENGDF LLRIEDTDQE    50
RQVEGALDII YNTLKMTGIH HDEGPDIGGP VGPYVQSERK HIYTEYAQKL 100
IDKGEAYYCF CTKERLDMLR ENSESLGRPH KYDKHCSHLS KEEIEENLKA 150
GIPFVIRQNN PNKGTTYFED EIFGKITVDN SELDDMVLIK SDGFPTYNFA 200
NVVDDHLMGI THVVRGSEYL SSSPKYNRLY DAFGWDVPKY IHCPPIMKDS 250
HSKLSKRNGD ASFQDLLEKG YLKDAILNYI ALLGWNPGTT EEIFSLEDLI 300
GKFDYKSINK APAIFDNKKL KWMNGEYIRM FSLEEFHKLA LPYYKGVITK 350
DFDLLKISEL LHTRTELLSE IPEQVDFFDE LPEYSCDLYV HKKMKTNFEN 400
SLESLEKALP VLESIDNWNT ETIHDSIMEL IKELEVKNGI VLWPIRTAVS 450
GKKFTPGGAF EIAEILGKEE TLKRVKIGIE KLKNEINQ 488
Length:488
Mass (Da):56,137
Last modified:December 15, 2003 - v2
Checksum:iFBB37A170846127B
GO

Sequence cautioni

The sequence AAO35565.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015927 Genomic DNA. Translation: AAO35565.1. Different initiation.
RefSeqiNP_781628.1. NC_004557.1.

Genome annotation databases

EnsemblBacteriaiAAO35565; AAO35565; CTC_00977.
GeneIDi1058871.
KEGGictc:CTC00977.
PATRICi19509821. VBICloTet101274_0959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015927 Genomic DNA. Translation: AAO35565.1 . Different initiation.
RefSeqi NP_781628.1. NC_004557.1.

3D structure databases

ProteinModelPortali Q896M5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 212717.CTC00977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO35565 ; AAO35565 ; CTC_00977 .
GeneIDi 1058871.
KEGGi ctc:CTC00977.
PATRICi 19509821. VBICloTet101274_0959.

Phylogenomic databases

eggNOGi COG0008.
KOi K01885.
OMAi KLEWYNG.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci CTET212717:GJAM-893-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Massachusetts / E88.

Entry informationi

Entry nameiSYE_CLOTE
AccessioniPrimary (citable) accession number: Q896M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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