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Q896M5

- SYE_CLOTE

UniProt

Q896M5 - SYE_CLOTE

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091ZincUniRule annotation
Metal bindingi111 – 1111ZincUniRule annotation
Metal bindingi136 – 1361ZincUniRule annotation
Metal bindingi138 – 1381ZincUniRule annotation
Binding sitei256 – 2561ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTET212717:GJAM-893-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CTC_00977
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001412: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Glutamate--tRNA ligasePRO_0000119546Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC00977.

Structurei

3D structure databases

ProteinModelPortaliQ896M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionAdd
BLAST
Motifi253 – 2575"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
KOiK01885.
OMAiKLEWYNG.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q896M5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGNKIRTRF APSPTGYMHV GNLRTALYAY LIAKHENGDF LLRIEDTDQE
60 70 80 90 100
RQVEGALDII YNTLKMTGIH HDEGPDIGGP VGPYVQSERK HIYTEYAQKL
110 120 130 140 150
IDKGEAYYCF CTKERLDMLR ENSESLGRPH KYDKHCSHLS KEEIEENLKA
160 170 180 190 200
GIPFVIRQNN PNKGTTYFED EIFGKITVDN SELDDMVLIK SDGFPTYNFA
210 220 230 240 250
NVVDDHLMGI THVVRGSEYL SSSPKYNRLY DAFGWDVPKY IHCPPIMKDS
260 270 280 290 300
HSKLSKRNGD ASFQDLLEKG YLKDAILNYI ALLGWNPGTT EEIFSLEDLI
310 320 330 340 350
GKFDYKSINK APAIFDNKKL KWMNGEYIRM FSLEEFHKLA LPYYKGVITK
360 370 380 390 400
DFDLLKISEL LHTRTELLSE IPEQVDFFDE LPEYSCDLYV HKKMKTNFEN
410 420 430 440 450
SLESLEKALP VLESIDNWNT ETIHDSIMEL IKELEVKNGI VLWPIRTAVS
460 470 480
GKKFTPGGAF EIAEILGKEE TLKRVKIGIE KLKNEINQ
Length:488
Mass (Da):56,137
Last modified:December 15, 2003 - v2
Checksum:iFBB37A170846127B
GO

Sequence cautioni

The sequence AAO35565.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA. Translation: AAO35565.1. Different initiation.
RefSeqiNP_781628.1. NC_004557.1.

Genome annotation databases

EnsemblBacteriaiAAO35565; AAO35565; CTC_00977.
GeneIDi1058871.
KEGGictc:CTC00977.
PATRICi19509821. VBICloTet101274_0959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA. Translation: AAO35565.1 . Different initiation.
RefSeqi NP_781628.1. NC_004557.1.

3D structure databases

ProteinModelPortali Q896M5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 212717.CTC00977.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO35565 ; AAO35565 ; CTC_00977 .
GeneIDi 1058871.
KEGGi ctc:CTC00977.
PATRICi 19509821. VBICloTet101274_0959.

Phylogenomic databases

eggNOGi COG0008.
KOi K01885.
OMAi KLEWYNG.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci CTET212717:GJAM-893-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Massachusetts / E88.

Entry informationi

Entry nameiSYE_CLOTE
AccessioniPrimary (citable) accession number: Q896M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: November 26, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3