Q896G3 (PROB_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	CTC_01044

Organism

Clostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]

Taxonomic identifier

212717 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium tetani

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family.
Sequence caution	The sequence AAO35627.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000109662
Regions
	Nucleotide binding	177 – 178	2	ATP By similarity
	Nucleotide binding	219 – 225	7	ATP By similarity
Sites
	Binding site	18	1	ATP By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	157	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q896G3 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: F6B2A67DEEC55699	FASTA	267	28,745
	10 20 30 40 50 60 MQFNRKEYLK DVKNIVVKVG SSTLTYENGL LNLFHIEHLV RQLVDLHNRG YNVILVSSGA 70 80 90 100 110 120 IGAGVGKLGL SEKPKSIPEK QAAAAVGQGI LLHTYEKIFA EYGKTIGQIL LTKEDMIDEV 130 140 150 160 170 180 RSCNATNTFN ALLDKRVIPI INENDAVVVD EIKVGDNDTL SAFVSKLVKA DLLILMSDIE 190 200 210 220 230 240 GLYSCDPRIN NDAELINFVE KITKDIISCA GGAGTDLGTG GMATKIKAAK IATSAGIPML 250 260 IVNGATNEVL QDVVEGKSVG TWFNAIK

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO35627.1. Different initiation.
RefSeq	NP_781690.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q896G3.
ModBase	Search...
Protein-protein interaction databases
STRING	212717.CTC01044.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO35627; AAO35627; CTC_01044.
GeneID	1059732.
KEGG	ctc:CTC01044.
PATRIC	19509955. VBICloTet101274_1026.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
KO	K00931.
OMA	LYSCDPR.
ProtClustDB	PRK12314.
Enzyme and pathway databases
BioCyc	CTET212717:GJAM-955-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_CLOTE

Accession

Primary (citable) accession number: Q896G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program