Q895M9 (RNC_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	CTC_01243

Organism

Clostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]

Taxonomic identifier

212717 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium tetani

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000180392
Regions
	Domain	7 – 134	128	RNase III
	Domain	161 – 230	70	DRBM
Sites
	Active site	51	1	Potential
	Active site	123	1	By similarity
	Metal binding	47	1	Magnesium By similarity
	Metal binding	120	1	Magnesium By similarity
	Metal binding	123	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q895M9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F28E1D35C65E3D6F	FASTA	235	27,387
	10 20 30 40 50 60 MNKYMDLKDL QNKIEIKFEN IKLLHIALTH SSYANEKKRI QYNERLEFLG DSVLQLVITE 70 80 90 100 110 120 YLFMNYKDKS EGELSKKRAL IVCENSLFSI AKNLELGEYI KMSKGEELTG GRERISILAD 130 140 150 160 170 180 AVEALIASIY LDKGIDVVRE FILKNFYNIV EKAMKDEIIL DYKTKLQEII QKDGDIQINY 190 200 210 220 230 ILIKHEGPPH RRKFYTEVKI KDNVLGYGIG YSKKESEQNA AKKAIENMEV NKEYE

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO35811.1.
RefSeq	NP_781874.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q895M9.
ModBase	Search...
Protein-protein interaction databases
STRING	212717.CTC01243.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO35811; AAO35811; CTC_01243.
GeneID	1059341.
KEGG	ctc:CTC01243.
PATRIC	19510435. VBICloTet101274_1265.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
KO	K03685.
OMA	LTHKSCK.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	CTET212717:GJAM-1140-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_CLOTE

Accession

Primary (citable) accession number: Q895M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program