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Protein

Ribonuclease 3

Gene

rnc

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47MagnesiumUniRule annotation1
Active sitei51UniRule annotation1
Metal bindingi120MagnesiumUniRule annotation1
Active sitei123UniRule annotation1
Metal bindingi123MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processmRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:CTC_01243
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001412 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001803921 – 235Ribonuclease 3Add BLAST235

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi212717.CTC01243.

Structurei

3D structure databases

ProteinModelPortaliQ895M9.
SMRiQ895M9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 134RNase IIIUniRule annotationAdd BLAST128
Domaini161 – 230DRBMUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the ribonuclease III family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZBM. Bacteria.
COG0571. LUCA.
KOiK03685.
OMAiLTHKSCK.
OrthoDBiPOG091H00R1.

Family and domain databases

CDDicd00593. RIBOc. 1 hit.
Gene3Di1.10.1520.10. 1 hit.
HAMAPiMF_00104. RNase_III. 1 hit.
InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
PfamiView protein in Pfam
PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q895M9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKYMDLKDL QNKIEIKFEN IKLLHIALTH SSYANEKKRI QYNERLEFLG
60 70 80 90 100
DSVLQLVITE YLFMNYKDKS EGELSKKRAL IVCENSLFSI AKNLELGEYI
110 120 130 140 150
KMSKGEELTG GRERISILAD AVEALIASIY LDKGIDVVRE FILKNFYNIV
160 170 180 190 200
EKAMKDEIIL DYKTKLQEII QKDGDIQINY ILIKHEGPPH RRKFYTEVKI
210 220 230
KDNVLGYGIG YSKKESEQNA AKKAIENMEV NKEYE
Length:235
Mass (Da):27,387
Last modified:June 1, 2003 - v1
Checksum:iF28E1D35C65E3D6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015927 Genomic DNA. Translation: AAO35811.1.
RefSeqiWP_011099473.1. NC_004557.1.

Genome annotation databases

EnsemblBacteriaiAAO35811; AAO35811; CTC_01243.
GeneIDi24254049.
KEGGictc:CTC_01243.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRNC_CLOTE
AccessioniPrimary (citable) accession number: Q895M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families