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Q895J3

- PNP_CLOTE

UniProt

Q895J3 - PNP_CLOTE

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi485 – 4851MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciCTET212717:GJAM-1177-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:CTC_01280
    OrganismiClostridium tetani (strain Massachusetts / E88)
    Taxonomic identifieri212717 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001412: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 705705Polyribonucleotide nucleotidyltransferasePRO_0000329604Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi212717.CTC01280.

    Structurei

    3D structure databases

    ProteinModelPortaliQ895J3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini552 – 61160KHUniRule annotationAdd
    BLAST
    Domaini621 – 68969S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    KOiK00962.
    OMAiINTENDG.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q895J3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHVLETDIA GRTLKVEYGK VGMLSNCAIL VSYGDTVVLI NANASKEPRD    50
    GIDFFPLSVE YEERLYSVGK IPGGFIKREG RPTENAILHA RAIDRPLRPL 100
    FPKGYRNDVQ IVCTVLSVES DNQPDILAMN GASLALCLSS IPFTTPVANV 150
    SVGLINGDFI LNPTQSQREE SILNLTVSAT KERVMMIEAG AEEVDEDTMY 200
    NAIRFGFEQC QKIVEFQEEA MRRFGKEKVE PVLYLVDKDI EKEVREFAFP 250
    MVKEAMYISD KDLRNEKIDE VKEKVKEEFE EKYPENDSDI SEALYKMQKE 300
    VVRNMILREK RRPDERAFDE IRKISCEVGL LPRTHGTGLF TRGLTQVMTA 350
    ATIGPLADVQ ILDSIEDEEF KRYMHHYNFP SYSVGETRPL RGPGRREIGH 400
    GALAEKALEP LIPSEEDFPY TIRLVSEVLS SNGSTSQASV CGSTLALLDA 450
    GVPIKRPAAG IAMGLVTNDD LTEEKILTDI QGIEDFFGDM DFKVAGTEKG 500
    ITALQVDTKI SGLSDNCIKE TLIKAKDARM YILGKIKECI PEHRAELSPY 550
    APRVYTMTIA PEKIRDVIGA GGKTINKIIG ETGVQIDIKE DGKIYVMSSD 600
    SVGANRALKM INDLTKDVEA GEIYLGKVTR TTNFGAFVEI LPGKEGLVHI 650
    SKLDFTRVNK VEDVVSVGDE ILVKVTDIDD QGRINLSRRD AIKKEEDEKT 700
    KENNH 705
    Length:705
    Mass (Da):78,551
    Last modified:June 1, 2003 - v1
    Checksum:i21A5F4DFD891BB4E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015927 Genomic DNA. Translation: AAO35847.1.
    RefSeqiNP_781910.1. NC_004557.1.
    WP_011099509.1. NC_004557.1.

    Genome annotation databases

    EnsemblBacteriaiAAO35847; AAO35847; CTC_01280.
    GeneIDi1059391.
    KEGGictc:CTC01280.
    PATRICi19510517. VBICloTet101274_1306.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015927 Genomic DNA. Translation: AAO35847.1 .
    RefSeqi NP_781910.1. NC_004557.1.
    WP_011099509.1. NC_004557.1.

    3D structure databases

    ProteinModelPortali Q895J3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 212717.CTC01280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO35847 ; AAO35847 ; CTC_01280 .
    GeneIDi 1059391.
    KEGGi ctc:CTC01280.
    PATRICi 19510517. VBICloTet101274_1306.

    Phylogenomic databases

    eggNOGi COG1185.
    KOi K00962.
    OMAi INTENDG.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci CTET212717:GJAM-1177-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Massachusetts / E88.

    Entry informationi

    Entry nameiPNP_CLOTE
    AccessioniPrimary (citable) accession number: Q895J3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3