Reviewed, UniProtKB/Swiss-Prot Q894B7 (MURE_CLOTE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	CTC_01632

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_0000101888
Regions
	Nucleotide binding	113 – 119	7	ATP Potential
	Region	155 – 156	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	405 – 408	4	Meso-diaminopimelate binding By similarity
	Motif	405 – 408	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	182	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	190	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	381	1	Meso-diaminopimelate By similarity
	Binding site	455	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	459	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	222	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q894B7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 47F2A9B9E8A2A7AC	FASTA	485	54,968
	10 20 30 40 50 60 MKLKEVLKKL EYNILNGNIE IDIENIQYDS RNIKKNDIFF AIQGYSTDGH KFIESAIEKG 70 80 90 100 110 120 AKVIVFDKGF ENENMYKDIT FIKVKNSRKA LAVAASNYYG NPKDKLKLIG VTGTNGKTTS 130 140 150 160 170 180 TFMIKSILEE AGFKVGLMGT ILNYIGDRKI YAQRTTPESL EIHKLFKDMV DSGVDYCVME 190 200 210 220 230 240 VSSHSLYLDR VYGIEFNEGI FTNLTQDHLD FHKTFENYYN SKLMLFKNSI NSVINIDDNY 250 260 270 280 290 300 GERILKEIEE KTFTYSIKKY SDLKAESVRL HSRGGEFTVD FKGNKEKINI HIPGEYNVSN 310 320 330 340 350 360 ALGSILACVN EGISLKVIKR GLEKLSGVPG RCEIVTMGYN LGYDVIVDYA HTPDGLDNVL 370 380 390 400 410 420 RTARDFTKGK LISVYGCGGD RDRAKRPIMG RIGTELSDLA ILTSDNPRTE EPFSIIDDIV 430 440 450 460 470 480 KGVTKDNYII VQSRREAIKK AMTIAKKDDV IVVAGKGHED YQILKDKTIH FDEREVIKEI IEELY

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO36175.1.
RefSeq	NP_782238.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1059017.
GenomeReviews	Gene locus CTC_01632 in contig AE015927_GR.
KEGG	ctc:CTC01632.
NMPDR	fig|212717.1.peg.1460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q894B7.
OMA	EDYLAHK.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_01632-MON.
BRENDA	6.3.2.13. 2082.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_CLOTE

Accession

Primary (citable) accession number: Q894B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)