Reviewed, UniProtKB/Swiss-Prot Q893Q9 (THIM_CLOTE)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	CTC_01752

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156930
Sites
	Metal binding	95	1	Magnesium 1 By similarity
	Metal binding	127	1	Magnesium 1 By similarity
	Binding site	46	1	Substrate; via amide nitrogen By similarity
	Binding site	122	1	ATP By similarity
	Binding site	173	1	ATP By similarity
	Binding site	200	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q893Q9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8C2291B24B27814B	FASTA	274	29,748
	10 20 30 40 50 60 MEIYKKIYNS LLKLKEKNPL IHHITNYVTV NDCANIVLAI GASPVMADDI REVEDMVSIA 70 80 90 100 110 120 SALVINIGTL NKKTVKSMFL AGKKANELNI PVILDPVGVG ATKYRTSIAL ELLEEIKFSV 130 140 150 160 170 180 VRGNISEVKV LSGMNASTKG VDAESWDETN PIEERIRLVK SLSEKYNAVI GITGEKDVIG 190 200 210 220 230 240 FKEEVYTVEN GSSIMAKITG AGCMCTSVIA SYCGAEENYL QATLSGVVSM GIAGEIAYEK 250 260 270 TKNLGPSSFR TALIDSIYNL EESSFNERGK VYNA

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO36283.1.
RefSeq	NP_782346.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1060928.
GenomeReviews	Gene locus CTC_01752 in contig AE015927_GR.
KEGG	ctc:CTC01752.
NMPDR	fig|212717.1.peg.1568.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q893Q9.
OMA	Q893Q9. ASPVMAH.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_01752-MON.
BRENDA	2.7.1.50. 2082.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_CLOTE

Accession

Primary (citable) accession number: Q893Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)