Q892X3 (PUR9_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	CTC_01962

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Bifunctional purine biosynthesis protein PurH HAMAP MF_00139		PRO_0000192086

Sequences

	Sequence		Length	Mass (Da)
	Q892X3 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 17F1CB10527AF3BB	FASTA	499	56,183
	10 20 30 40 50 60 MIKRALISVY NKEGILEFAK FLEKNHVEII STGGTYKHLK DNCVRVKEVS QITNFDEILD 70 80 90 100 110 120 GRVKTLHPAV HGGILAIRDN KEHMDTIKDK NIEPIDMVIV NLYPFFEKIG KGLSLEEQIE 130 140 150 160 170 180 FIDIGGPTMI RAAAKNFKDV VVITDPKDYK RIEEKLEKED EIDFNNRKTL AGKVFNLMSA 190 200 210 220 230 240 YDGAISKFLL EEEYPEYLSL SYKKGKDLRY GENPHQTAAF YTSLTEDGAF KDFEKLNGKE 250 260 270 280 290 300 LSYNNIKDMD VAWKIVGEFE EIACCALKHN SPCGVAIGDT LLESYKKAYE CDPISIFGGI 310 320 330 340 350 360 VAFNRKVNKE TAKELVKIFL EVIIAPDYDE DALRILKGKK NLRVIKCNTK PLDKMEICKV 370 380 390 400 410 420 DVGILVQSTD NALIKDMDVV TAKKPTEEEM ENMIFAMKVV KYVKSNAIVV VKDKKAVGIA 430 440 450 460 470 480 GGQVNRIWAA CQALERGNNS VVLASDAFFP FDDVVKKASE YGIKAIIQPG GSIRDKDSIE 490 ECDEKGISMV FTGIRHFKH

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO36469.1.
RefSeq	NP_782532.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q892X3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1059924.
GenomeReviews	Gene locus CTC_01962 in contig AE015927_GR.
KEGG	ctc:CTC01962.
NMPDR	fig|212717.1.peg.1754.
PATRIC	19511975. VBICloTet101274_2035.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG498048.
OMA	FTGTRHF.
PhylomeDB	Q892X3.
ProtClustDB	PRK00881.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_01962-MONOMER.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.140.20. G3DSA:3.40.140.20. 2 hits. G3DSA:3.40.50.1380. MGS-like_dom. 1 hit.
KO	K00602.
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. PurH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_CLOTE

Accession

Primary (citable) accession number: Q892X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program