Q891R3 (FTHS_CLOTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	CTC_02304

Organism

Clostridium tetani (strain Massachusetts / E88) [Complete proteome] [HAMAP]

Taxonomic identifier

212717 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium tetani

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199341
Regions
	Nucleotide binding	68 – 75	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q891R3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5FF7F0F0943AF04B	FASTA	559	60,073
	10 20 30 40 50 60 MEKVLTDIEI AQKAQMKPIG EIAEKYGILE DELELYGKYK AKLSLDIFDR LKDEKDGKLV 70 80 90 100 110 120 LVTAISPTPA GEGKSTTSIG LGQALNKIGK KTFIALREPS LGPVFGVKGG AAGGGYAQVV 130 140 150 160 170 180 PMEDINLHFT GDMHAIGITN NLLSAAIDNH IHQGNALKID SREIVWKRVV DMNDRALRNV 190 200 210 220 230 240 VVGMGGKACG FTRQDGFMIT VASEVMAILC LAKDLMDLKE RLGNIIVAYS LEGKPVTAGD 250 260 270 280 290 300 LKVNGAMAML LKDAIKPNIV QTLENTPALI HGGPFANIAH GCNSLIATRL GLKLGDILVT 310 320 330 340 350 360 EAGFGADLGA EKFLDIKCRY GGLKPDAVVI VATIRALKMH GGVKKTELSG ENLEALDKGF 370 380 390 400 410 420 ANLQKHITNM KNFGLPVMVA VNRFITDSEA EIDLLIKKCK EIGVEVSLNE VWAKGGEGGI 430 440 450 460 470 480 EMAEKLVKIL ETEKPNYKPL YDVEDSIPEK LNKIVKELYG GEGVVIESSA MKQIKKLEEI 490 500 510 520 530 540 GLDKLPICMA KTQFSFSDDA TLMGAPKGFN ITIKNVRVSA GAGFIVCETG NIMVMPGLPK 550 VPAAEKMDVD ENGNISGLF

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015927 Genomic DNA. Translation: AAO36782.1.
RefSeq	NP_782845.1. NC_004557.1.
3D structure databases
ProteinModelPortal	Q891R3.
SMR	Q891R3. Positions 7-557.
ModBase	Search...
Protein-protein interaction databases
STRING	212717.CTC02304.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO36782; AAO36782; CTC_02304.
GeneID	1060481.
KEGG	ctc:CTC02304.
PATRIC	19512709. VBICloTet101274_2397.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
KO	K01938.
OMA	HEQTAST.
Enzyme and pathway databases
BioCyc	CTET212717:GJAM-2117-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CLOTE

Accession

Primary (citable) accession number: Q891R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program