Reviewed, UniProtKB/Swiss-Prot Q891L4 (KHSE_CLOTE)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	CTC_02357

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Homoserine kinase HAMAP MF_00384		PRO_0000156562
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q891L4-1 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: E9D0386477A9C257	FASTA	300	33,472
	10 20 30 40 50 60 MLEIKVPGTS ANFGPGFDSL GVALSIYNKF YVEEIESGIY IEGCEEKYRN DKNLFYVSMK 70 80 90 100 110 120 KVFDKFGYKL KGLRIKIEGD IPESRGLGSS ASCIVGGVMA ANDFIGNKLS KEEMLEISTE 130 140 150 160 170 180 IEGHPDNVAP AIFGGMTAVV KDKDRIYHDK IDIDKNINFH ALIPNFILST KKSREVLPKT 190 200 210 220 230 240 VDYKDAVFNV GRVALMISSL ISGKYENLEV AFEDKLHQPY RKELIGNYDD LIVKCKKNGA 250 260 270 280 290 300 LGVFLSGAGP TIMAITEKHR EDFLKNMNEE LRILNHKWNI KKLVVDHKGA TVISKLNGGI

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO36831.1. Different initiation.
RefSeq	NP_782894.1.
3D structure databases
HSSP	HSSP built from PDB template 1H74 based on UniProtKB Q58504.
ModBase	Search...
Genome annotation databases
GeneID	1060525.
GenomeReviews	Gene locus CTC_02357 in contig AE015927_GR.
KEGG	ctc:CTC02357.
NMPDR	fig|212717.1.peg.2116.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q891L4.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_02357-MON.
BRENDA	2.7.1.39. 2082.
Family and domain databases
HAMAP	MF_00384. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR014721. Ribosomal_S5_D2-type_fold. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CLOTE

Accession

Primary (citable) accession number: Q891L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)