Reviewed, UniProtKB/Swiss-Prot Q891B8 (GLPK2_CLOTE)

Names and origin

Protein names

Recommended name:
    Glycerol kinase 2
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase 2
    Glycerokinase 2
      Short name=GK 2

Gene names

Name:	glpK2
Ordered Locus Names:	CTC_02462

Organism

Clostridium tetani [Complete proteome] [HAMAP]

Taxonomic identifier

1513 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Glycerol kinase 2 HAMAP MF_00186		PRO_0000059446
Regions
	Nucleotide binding	409 – 413	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q891B8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2663640F6C2A55C4	FASTA	493	55,340
	10 20 30 40 50 60 MEEYIMVLDQ GSTRSKCILF DKKGEVLGES EREVEPIYPK LGWMEYNPVN IWASQFSVTT 70 80 90 100 110 120 ELLAKYNISI EKIASIGITN QRETVLVWDK RTGIPVYNAI AWQCRRTVEI SEELNKKGYG 130 140 150 160 170 180 KVIKEKTGLV LDPYFSATKI KWILDNVEGA RKEAERGNLA FGTMDSWLIW NLTKGEKHMT 190 200 210 220 230 240 DYSNASRTML FNIHTLQWDE ELLEIFQIPK SMLPEVGPSS HVYGHTDSIL FGKEVPIGGV 250 260 270 280 290 300 AGDQNSSLFG QTAFEEGAAK NTYGTGCFML MNTGNKPINS QKGLITTIAW GLDGEVTYAL 310 320 330 340 350 360 EGSVFMAGEG INWIKDNLRM IDSLEDAEEY ALSVEDTNNV YMVPAFGGLG APYWNPEAKG 370 380 390 400 410 420 VVIGLTRGSK KEHFIRAVIE SLAYQSYDVL KAMEEDSGIV LKNLRVDGQY SVNNFLMQFQ 430 440 450 460 470 480 SDILNCKVEK PKNILNIAGL GAAYFAGLAV GFWKNKEDIL KNNGIEKEFN SSIDDRRRKV 490 LVEGWKEAIK RTI

References

[1]

"The genome sequence of Clostridium tetani, the causative agent of tetanus disease." Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H., Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A., Gottschalk G. Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003) [PubMed: 12552129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Massachusetts / E88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015927 Genomic DNA. Translation: AAO36927.1.
RefSeq	NP_782990.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1060626.
GenomeReviews	Gene locus CTC_02462 in contig AE015927_GR.
KEGG	ctc:CTC02462.
NMPDR	fig|212717.1.peg.2212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q891B8.
OMA	Q891B8. GESEREV.
Enzyme and pathway databases
BioCyc	CTET212717:CTC_02462-MON.
BRENDA	2.7.1.30. 2082.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK2_CLOTE

Accession

Primary (citable) accession number: Q891B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	May 5, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)