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Protein

Glutamate 5-kinase

Gene

proB

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATPUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Binding sitei158Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179ATPUniRule annotation2
Nucleotide bindingi220 – 226ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLPLA220668:G1GW0-14-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:lp_0016
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000432 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096831 – 265Glutamate 5-kinaseAdd BLAST265

Interactioni

Protein-protein interaction databases

STRINGi220668.lp_0016

Structurei

3D structure databases

SMRiQ890J5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931

Family and domain databases

Gene3Di3.40.1160.101 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q890J5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVIRKLKAK RVVIKVGTST LVYPDGSINL RAIDQLAFVI SAMRHRGREV
60 70 80 90 100
VLVSSGAIGV GLNYMGLKQR PKAIPEQQAI AAIGQTELIS IYKERFAIYE
110 120 130 140 150
QKIGQLLLTR DIFVYPKSHH NVLNTFEALL KQNVVPIVNE NDTVAVDELD
160 170 180 190 200
HETKFGDNDQ LSAIVATNIG ADLLIMLSDI DGFYDGNPNA DARAQLLGHI
210 220 230 240 250
EKVDQHTYKL AGGSGSRFGT GGMVTKLKAA ERMIDANGQM ILANGADPTI
260
IFQILAGEPV GTLFG
Length:265
Mass (Da):28,796
Last modified:November 16, 2011 - v2
Checksum:i160B92EA661C9A29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA Translation: CCC77593.1
RefSeqiWP_003643611.1, NC_004567.2
YP_004888107.1, NC_004567.2

Genome annotation databases

EnsemblBacteriaiCCC77593; CCC77593; lp_0016
GeneIDi1061284
KEGGilpl:lp_0016
PATRICifig|220668.9.peg.14

Similar proteinsi

Entry informationi

Entry nameiPROB_LACPL
AccessioniPrimary (citable) accession number: Q890J5
Secondary accession number(s): F9US46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: November 16, 2011
Last modified: February 28, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome