Reviewed, UniProtKB/Swiss-Prot Q890C2 (THIM_LACPL)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	lp_0113

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156940
Sites
	Metal binding	90	1	Magnesium 1 By similarity
	Metal binding	122	1	Magnesium 1 By similarity
	Binding site	41	1	Substrate; via amide nitrogen By similarity
	Binding site	117	1	ATP By similarity
	Binding site	163	1	ATP By similarity
	Binding site	190	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q890C2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: CC4E65F7DC6FF44F	FASTA	263	27,521
	10 20 30 40 50 60 MELQLLNTLR NHNPIVFNIA NFVTVQDVAN GLNALGASPI MSAEVQEAET MVQIAGAVCI 70 80 90 100 110 120 NLGTLTTTQI DQMRVVGKLA NQYHKPVVLD PVAVGAVPYR KEVALALLSD FQVDVIRGNA 130 140 150 160 170 180 GEIAALAGMD WQAKGIDAGS GQGDLVEIAQ ACAQQFQCCV ILSGPTDVIT DGQRVVKVAN 190 200 210 220 230 240 GTPLFQRHVG SGDLLSSIVA AFTAVSPTDV YQAAQVACLV LAGAGELVAS QLTADRPATF 250 260 AIDLIDRLSL VTVSEIQTIA KVD

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL935252 Genomic DNA. Translation: CAD62792.1.
RefSeq	NP_783954.1.
3D structure databases
HSSP	HSSP built from PDB template 1EKQ based on UniProtKB P39593.
ModBase	Search...
Genome annotation databases
GeneID	1061358.
GenomeReviews	Gene locus lp_0113 in contig AL935263_GR.
KEGG	lpl:lp_0113.
NMPDR	fig|220668.1.peg.88.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q890C2.
OMA	Q890C2. ASPVMAH.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_0113-MON.
BRENDA	2.7.1.50. 593.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
ProtoNet	Search...

Entry information

Entry name

THIM_LACPL

Accession

Primary (citable) accession number: Q890C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)