Q88Z84 (KPRS1_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 1
Short name=RPPK 1
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 1
Short name=P-Rib-PP synthase 1
Short name=PRPP synthase 1

Gene names

Name:	prs1
Ordered Locus Names:	lp_0471

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus plantarum

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP-Rule MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Ribose-phosphate pyrophosphokinase 1 HAMAP-Rule MF_00583_B		PRO_0000141148
Regions
	Region	218 – 231	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q88Z84 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 28AC04BEAADCD656	FASTA	326	35,880
	10 20 30 40 50 60 MSEQYFDPKL KIFALNSNKP LAEKIADAVG VKLGKTSVDR FSDGEIRINI EESIRGDQVY 70 80 90 100 110 120 IIQSTSAPVN DNLMELLIMI DALRRASAKT INVVIPYYGY ARQDRKARSR EPITAKLVAN 130 140 150 160 170 180 MLETAGATRI LALDLHAAQI QGFFDIPLDH LMGAPLLADY FLNHHLDENA VVVSPDHGGV 190 200 210 220 230 240 TRARKLAEFL KAPIAIIDKR RPRANVAEVM NIIGDVKGKR AIMIDDMIDT AGTITLGAQA 250 260 270 280 290 300 LVDAGATEVY ASCTHPVLSG PAIERIEKSP IKKLVVTDSI ELPAAKRIDK IEQVSVGQLM 310 320 GQAIKFIHEN KPVSPLFKNR FHNEEN

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL935263 Genomic DNA. Translation: CCC77970.1.
RefSeq	YP_004888484.1. NC_004567.2.
3D structure databases
ProteinModelPortal	Q88Z84.
SMR	Q88Z84. Positions 2-317.
ModBase	Search...
Protein-protein interaction databases
STRING	220668.lp_0471.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC77970; CCC77970; lp_0471.
GeneID	1061409.
KEGG	lpl:lp_0471.
PATRIC	22247401. VBILacPla27411_0387.
Phylogenomic databases
eggNOG	COG0462.
HOGENOM	HOG000210449.
KO	K00948.
OMA	YVQIQES.
ProtClustDB	PRK01259.
Enzyme and pathway databases
UniPathway	UPA00087; UER00172.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B.
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase_dom. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_LACPL

Accession

Primary (citable) accession number: Q88Z84
Secondary accession number(s): F9UKV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program