Reviewed, UniProtKB/Swiss-Prot Q88Z84 (KPRS1_LACPL)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 1
      Short name=RPPK 1
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 1
      Short name=P-Rib-PP synthetase 1
      Short name=PRPP synthetase 1

Gene names

Name:	prs1
Ordered Locus Names:	lp_0471

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Ribose-phosphate pyrophosphokinase 1 HAMAP MF_00583		PRO_0000141148
Regions
	Region	218 – 231	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q88Z84-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 28AC04BEAADCD656	FASTA	326	35,880
	10 20 30 40 50 60 MSEQYFDPKL KIFALNSNKP LAEKIADAVG VKLGKTSVDR FSDGEIRINI EESIRGDQVY 70 80 90 100 110 120 IIQSTSAPVN DNLMELLIMI DALRRASAKT INVVIPYYGY ARQDRKARSR EPITAKLVAN 130 140 150 160 170 180 MLETAGATRI LALDLHAAQI QGFFDIPLDH LMGAPLLADY FLNHHLDENA VVVSPDHGGV 190 200 210 220 230 240 TRARKLAEFL KAPIAIIDKR RPRANVAEVM NIIGDVKGKR AIMIDDMIDT AGTITLGAQA 250 260 270 280 290 300 LVDAGATEVY ASCTHPVLSG PAIERIEKSP IKKLVVTDSI ELPAAKRIDK IEQVSVGQLM 310 320 GQAIKFIHEN KPVSPLFKNR FHNEEN

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL935253 Genomic DNA. Translation: CAD63098.1.
RefSeq	NP_784259.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKU based on UniProtKB P14193.
SMR	Q88Z84. Positions 2-317.
ModBase	Search...
Genome annotation databases
GeneID	1061409.
GenomeReviews	Gene locus lp_0471 in contig AL935263_GR.
KEGG	lpl:lp_0471.
NMPDR	fig|220668.1.peg.393.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q88Z84.
OMA	NVAEIMN.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_0471-MON.
BRENDA	2.7.6.1. 593.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_LACPL

Accession

Primary (citable) accession number: Q88Z84

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)