Q88Z14 (NADE_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	lp_0566

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077568
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity
Sites
	Active site	49	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88Z14 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A1C78763ED7949D5	FASTA	275	30,590
	10 20 30 40 50 60 MRPLQAEIIK ALHVAPTIDP EVEIRRSIDF LKAYLTKNTF LKTYVLGISG GQDSTLAGKL 70 80 90 100 110 120 TEMAITEMRQ ETGDDRYQFI AVRLPYGNQA DEADAMAAID FMQADVTDRV DIQPATDAMV 130 140 150 160 170 180 TALEANQLTI HDFNKGNIKA RQRMIVQYGI AGEMHGAVVG TDHAAEAVTG FYTKYGDGGA 190 200 210 220 230 240 DIVPLWRLNK RQGKQMLAAL DAPKHLYDKV PTADLEEDRP ALPDEVALGV RYDDIDDYLE 250 260 270 GRTVSDAAAE KIEAWYLKTA HKRHAAITVF DDFWK

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL935263 Genomic DNA. Translation: CCC78052.1.
RefSeq	NP_784340.1. NC_004567.1.
3D structure databases
HSSP	HSSP built from PDB template 1WXI based on UniProtKB P18843.
ProteinModelPortal	Q88Z14.
SMR	Q88Z14. Positions 4-275.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064235.
GenomeReviews	Gene locus lp_0566 in contig AL935263_GR.
KEGG	lpl:lp_0566.
NMPDR	fig|220668.1.peg.474.
PATRIC	22247585. VBILacPla27411_0470.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_0566-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_LACPL

Accession

Primary (citable) accession number: Q88Z14
Secondary accession number(s): F9UL37

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program