Reviewed, UniProtKB/Swiss-Prot Q88YZ4 (FABH1_LACPL)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1
    EC=2.3.1.180
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III protein 1
    Beta-ketoacyl-ACP synthase III 1
      Short name=KAS III 1

Gene names

Name:	fabH1
Ordered Locus Names:	lp_0588

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1 HAMAP MF_01815		PRO_0000110437
Regions
	Region	255 – 259	5	ACP-binding By similarity
Sites
	Active site	114	1	By similarity
	Active site	254	1	By similarity
	Active site	284	1	By similarity
Experimental info
	Sequence conflict	58	1	T → A in BAA93641. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q88YZ4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 83C4C6B3DA71427D	FASTA	323	34,391
	10 20 30 40 50 60 MPTYTKLTAM GQYVPGRVVD NDELAAIMDT SDEWIQAHTG IKTRHYAMND ENTSDLATQV 70 80 90 100 110 120 AQQLLQKSGL AASAIDLILV TTITPDALTP ATACLVQANI GADNAFAFDL SAACAGFTFG 130 140 150 160 170 180 LATADKFIRS GQYQNVMVIS AEVNSKMMDF QDRTAAVFFG DGAGGALLQA TDNPDENSLI 190 200 210 220 230 240 AEKLESQGNA TVIHSGRVRP ITEVAATNYP QTDAFYQVGR DVFQFATTVV PEQMRGLIAS 250 260 270 280 290 300 AQLTPSDLQY VICHQANLRI IEKIAANLAL PMTKFPHNVD HYGNTSSAGV AMALANVFDT 310 320 LTGPVLLTAF GGGLAYGSVL IKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of Lactobacillus plantarum genes for beta-ketoacyl-acyl carrier protein synthase III(fabH) and acetyl coenzyme A carboxylase (accBCDA), which are essential for fatty acid biosynthesis." Kiatpapan P., Kobayashi H., Sakaguchi M., Ono H., Yamashita M., Kaneko Y., Murooka Y. Appl. Environ. Microbiol. 67:426-433(2001) [PubMed: 11133475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: L137.
[2]	"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025973 Genomic DNA. Translation: BAA93641.1. AL935253 Genomic DNA. Translation: CAD63202.1.
RefSeq	NP_784361.1.
3D structure databases
SMR	Q88YZ4. Positions 7-322.
ModBase	Search...
Genome annotation databases
GeneID	1061551.
KEGG	lpl:lp_0588.
NMPDR	fig|220668.1.peg.495.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	SADEIDC.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_0588-MONOMER.
BRENDA	2.3.1.180. 593.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH1_LACPL

Accession

Primary (citable) accession number: Q88YZ4
Secondary accession number(s): Q9LCH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)