Reviewed, UniProtKB/Swiss-Prot Q88XZ7 (SYS2_LACPL)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase 2
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase 2
    Serine--tRNA ligase 2
      Short name=SerRS 2

Gene names

Name:	serS2
Ordered Locus Names:	lp_1012

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Seryl-tRNA synthetase 2 HAMAP MF_00176		PRO_0000122066
Regions
	Nucleotide binding	261 – 263	3	ATP By similarity
	Nucleotide binding	348 – 351	4	ATP By similarity
	Region	230 – 232	3	Serine binding By similarity
Sites
	Binding site	284	1	Serine By similarity
	Binding site	383	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88XZ7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2F44FE6016E5CEF6	FASTA	425	48,136
	10 20 30 40 50 60 MLDVKMIRQN PDFVKEQLGH RGVAASDIDD LLAADEKRRE LIAKSEQLKS TRNQVSGEIS 70 80 90 100 110 120 QKKRNKEDAS AEIAEMQKVS ADVKTLDEER RTVDAQVQDM AAHLPNMPHP DVPVSLKEED 130 140 150 160 170 180 AVELRRIGTP RHFDFTPKAH WDIGEDLGIL DFERGAKVSG SRFLYYVGDG AKLERAVYNF 190 200 210 220 230 240 FLDQHEAEGY TEVLPPYMVT DESMYGTGQF PKFKEDAFRI TTQDLTLIPT AEVPLVNYYR 250 260 270 280 290 300 DEVIPAEKLP VYFTALSPAF REEAGSAGRD TKGLIRLHQF NKVEMVKFTK PEDSWDELEK 310 320 330 340 350 360 LTNNAESLLK KLGLPYHVIT LTTGDMSFTA AMTHDLEVWF PEQDKYREIS SCSNCTDFQA 370 380 390 400 410 420 RRAHIQYRDD DGKLQFVHTL NGSGLAVGRT VAAILENYQN EDGTVTIPDV LVPYMHGKTK IEKQG

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL935254 Genomic DNA. Translation: CAD63560.1.
RefSeq	NP_784713.1.
3D structure databases
HSSP	HSSP built from PDB template 1SER based on UniProtKB P34945.
ModBase	Search...
Genome annotation databases
GeneID	1063430.
GenomeReviews	Gene locus lp_1012 in contig AL935263_GR.
KEGG	lpl:lp_1012.
NMPDR	fig|220668.1.peg.847.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q88XZ7.
OMA	KEALNNR.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_1012-MON.
BRENDA	6.1.1.11. 593.
Family and domain databases
HAMAP	MF_00176. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS2_LACPL

Accession

Primary (citable) accession number: Q88XZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)