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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44S-adenosyl-L-methionineUniRule annotation1
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Active sitei118By similarity1
Binding sitei118S-adenosyl-L-methionineUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei154SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLPLA220668:G137Z-1243-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:lp_1458
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000432 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713391 – 214tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST214

Interactioni

Protein-protein interaction databases

STRINGi220668.lp_1458.

Structurei

3D structure databases

ProteinModelPortaliQ88WZ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 129Interaction with RNAUniRule annotation6
Regioni192 – 195Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88WZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRNKPWAP KLIAAHPELI TEDPTQLKGR WQSRFAKPQP LQIEVGSGKG
60 70 80 90 100
QFIIEMAKRH PEINYVAIEI QTSVIAIILK KLVEAPLPNL QLAHADGQAV
110 120 130 140 150
TAFFEPHEVD RLYLNFSDPW PKSRHEKRRL TYKSFLSSYR EVLKPNGQIE
160 170 180 190 200
FKTDNRGLFE FSLTSMNNFG MQFEQVWLDL HAVATPEDNV ETEYEQKFSA
210
AGPIYKIIAT FPAK
Length:214
Mass (Da):24,606
Last modified:June 16, 2003 - v1
Checksum:iD55FCF8C1D549532
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC78788.1.
RefSeqiWP_003644279.1. NC_004567.2.
YP_004889302.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC78788; CCC78788; lp_1458.
GeneIDi1062373.
KEGGilpl:lp_1458.
PATRICi22249157. VBILacPla27411_1221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC78788.1.
RefSeqiWP_003644279.1. NC_004567.2.
YP_004889302.1. NC_004567.2.

3D structure databases

ProteinModelPortaliQ88WZ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220668.lp_1458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC78788; CCC78788; lp_1458.
GeneIDi1062373.
KEGGilpl:lp_1458.
PATRICi22249157. VBILacPla27411_1221.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciLPLA220668:G137Z-1243-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_LACPL
AccessioniPrimary (citable) accession number: Q88WZ9
Secondary accession number(s): F9UNJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.