Q88WA8 (SYN2_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase 2
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase 2
Short name=AsnRS 2

Gene names

Name:	asnS2
Ordered Locus Names:	lp_1740

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Asparagine--tRNA ligase 2 HAMAP MF_00534		PRO_0000176419

Sequences

	Sequence		Length	Mass (Da)
	Q88WA8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C2A3AAEDFD02DA67	FASTA	432	49,868
	10 20 30 40 50 60 MVQQINIIDA KDHVDEKVKI GAWLTNKRSS GKIAFLQLRD GSAFFQGVVV KSQVSEEVFE 70 80 90 100 110 120 LAKEVKQEAS MWITGVIHED SRSKFGYEIE VEDIVVVGES EEYPITPKEH GVDFLLDHRH 130 140 150 160 170 180 LWLRSKRPWA IMNIRNEVIR GTYEFFNQEG FIKMDSPILT GSAPEGTTEL FHTEYFDRDA 190 200 210 220 230 240 YLSQSGQLYA EAGALAYGKV FTFGPTFRAE KSKTRRHLIE FWMIEPEMAF MHQEQSLEVQ 250 260 270 280 290 300 ERYIAFLVQG VIDHCQYALD ILGRDVETLK KYTKLPYPRI SYDEAIELLK ENDFDVDWGV 310 320 330 340 350 360 DFGSPEETFL ADHFDQPVFV LNYPKAIKPF YMKPHPTRDD VVICADLLAP EGYGEIIGGS 370 380 390 400 410 420 ERATDYNYLL DQIKQAGLNP DDYAWYLDLR KYGSVPHAGF GLGLERFLTW ITAEDHVRET 430 IPFPRLLNRI YP

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL935263 Genomic DNA. Translation: CCC79034.1.
RefSeq	NP_785313.1. NC_004567.1.
3D structure databases
ProteinModelPortal	Q88WA8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064108.
GenomeReviews	Gene locus lp_1740 in contig AL935263_GR.
KEGG	lpl:lp_1740.
NMPDR	fig|220668.1.peg.1447.
PATRIC	22249653. VBILacPla27411_1468.
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AVDYPIT.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_1740-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN2_LACPL

Accession

Primary (citable) accession number: Q88WA8
Secondary accession number(s): F9UP95

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program