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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciLPLA220668-WGS:GSPK-1584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid, gidC
Ordered Locus Names:lp_1848
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000432 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117248Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi220668.lp_1848.

Structurei

3D structure databases

ProteinModelPortaliQ88W23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88W23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIPTVNVI GAGLAGSEAA WHIANMGVNV RLYEMRPNKM TPAHHTAQFA
60 70 80 90 100
ELVCTNSLRA NQLANAAGLL KAEMRQMDSI VMQAAEHHAV PAGGALAVDR
110 120 130 140 150
DTFSAEITAA ITALPNVEII NEEITSLPDG ITVVATGPLT AASLAKSIQA
160 170 180 190 200
FNDEDDLHFF DAAAPILTKD SIDMDKVYLK SRYDRGEAAY LNCPMTEAEF
210 220 230 240 250
DVFYDALIHA EMAEAHDFEN SDVFEGCMPI EVMAQRGRQT MLFGPLKPVG
260 270 280 290 300
LEDPKTGKQP FAVVQLRQDD AAGDLYNIVG FQTHLKWGEQ KRVFSLIPGL
310 320 330 340 350
ENVEFVRYGV MHRNTFMKSP KLLTPTYQTQ QRPDLFFAGQ MTGVEGYIES
360 370 380 390 400
AASGIVAGTN AARLALGLEP VVFPTDTMMG AMAHYITHTS ASNFQPMNAN
410 420 430 440
FGIMPKLKQR IRDKRERNTA ISERALADLT TFKDETLTVN N
Length:441
Mass (Da):48,261
Last modified:June 1, 2003 - v1
Checksum:i0D1B4E9B6DEDF78D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC79121.1.
RefSeqiWP_003645028.1. NC_004567.2.
YP_004889635.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC79121; CCC79121; lp_1848.
GeneIDi1064052.
KEGGilpl:lp_1848.
PATRICi22249833. VBILacPla27411_1558.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC79121.1.
RefSeqiWP_003645028.1. NC_004567.2.
YP_004889635.1. NC_004567.2.

3D structure databases

ProteinModelPortaliQ88W23.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220668.lp_1848.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC79121; CCC79121; lp_1848.
GeneIDi1064052.
KEGGilpl:lp_1848.
PATRICi22249833. VBILacPla27411_1558.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Enzyme and pathway databases

BioCyciLPLA220668-WGS:GSPK-1584-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Entry informationi

Entry nameiTRMFO_LACPL
AccessioniPrimary (citable) accession number: Q88W23
Secondary accession number(s): F9UPI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: January 20, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.