Reviewed, UniProtKB/Swiss-Prot Q88W23 (TRMFO_LACPL)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid, gidC
Ordered Locus Names:	lp_1848

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000117248
Regions
	Nucleotide binding	11 – 16	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88W23-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0D1B4E9B6DEDF78D	FASTA	441	48,261
	10 20 30 40 50 60 MASIPTVNVI GAGLAGSEAA WHIANMGVNV RLYEMRPNKM TPAHHTAQFA ELVCTNSLRA 70 80 90 100 110 120 NQLANAAGLL KAEMRQMDSI VMQAAEHHAV PAGGALAVDR DTFSAEITAA ITALPNVEII 130 140 150 160 170 180 NEEITSLPDG ITVVATGPLT AASLAKSIQA FNDEDDLHFF DAAAPILTKD SIDMDKVYLK 190 200 210 220 230 240 SRYDRGEAAY LNCPMTEAEF DVFYDALIHA EMAEAHDFEN SDVFEGCMPI EVMAQRGRQT 250 260 270 280 290 300 MLFGPLKPVG LEDPKTGKQP FAVVQLRQDD AAGDLYNIVG FQTHLKWGEQ KRVFSLIPGL 310 320 330 340 350 360 ENVEFVRYGV MHRNTFMKSP KLLTPTYQTQ QRPDLFFAGQ MTGVEGYIES AASGIVAGTN 370 380 390 400 410 420 AARLALGLEP VVFPTDTMMG AMAHYITHTS ASNFQPMNAN FGIMPKLKQR IRDKRERNTA 430 440 ISERALADLT TFKDETLTVN N

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL935257 Genomic DNA. Translation: CAD64248.1.
RefSeq	NP_785399.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1064052.
GenomeReviews	Gene locus lp_1848 in contig AL935263_GR.
KEGG	lpl:lp_1848.
NMPDR	fig|220668.1.peg.1533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q88W23.
OMA	Q88W23. MKPVGLT.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_1848-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_LACPL

Accession

Primary (citable) accession number: Q88W23

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)