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Q88VY1 (PFKA_LACPL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfk
Ordered Locus Names:lp_1898
OrganismLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]
Taxonomic identifier220668 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000111959

Regions

Nucleotide binding72 – 732ATP By similarity
Nucleotide binding102 – 1054ATP By similarity
Region125 – 1273Substrate binding By similarity
Region169 – 1713Substrate binding By similarity
Region249 – 2524Substrate binding By similarity

Sites

Active site1271Proton acceptor By similarity
Metal binding1031Magnesium; catalytic By similarity
Binding site111ATP; via amide nitrogen By similarity
Binding site1621Substrate; shared with dimeric partner By similarity
Binding site2221Substrate By similarity
Binding site2431Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88VY1 [UniParc].

Last modified June 16, 2003. Version 1.
Checksum: 8DD32DD469261668

FASTA32034,266
        10         20         30         40         50         60 
MKRIGILTSG GDAPGMNAAV RAVAGKAMAE GLEAYGINYG FAGLVAGDIH KIEAADLDGV 

        70         80         90        100        110        120 
IQRGGTLLYS ARYPEFAHEE GQLKGIEQLK RFGIDALVVI GGDGSYHGAL RLTEHGYNTI 

       130        140        150        160        170        180 
GLPGTIDNDI PYTDFTIGFD TAVNTNVQAL DRIYDTAHSH DRTFVVEVMG RGAGDVALWS 

       190        200        210        220        230        240 
GVSIGATAIV VPEVDWDMEE IANKIKHNRA NGHRSNLIVL AEGVMGAQEF VEKLSEYGDF 

       250        260        270        280        290        300 
DARGNTIAHM QRGGNPTAKD RVMASKMGAY AVELLLAGKG GLAVGIQNNQ LVNHNILDLF 

       310        320 
ESKHDVEVSL DKLNEEISFK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL935263 Genomic DNA. Translation: CCC79163.1.
RefSeqYP_004889677.1. NC_004567.2.

3D structure databases

ProteinModelPortalQ88VY1.
SMRQ88VY1. Positions 1-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING220668.lp_1898.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCC79163; CCC79163; lp_1898.
GeneID1064199.
KEGGlpl:lp_1898.
PATRIC22249915. VBILacPla27411_1599.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAYRRGKLH.

Enzyme and pathway databases

BioCycLPLA220668-WGS:GSPK-1625-MONOMER.
SABIO-RKQ88VY1.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_LACPL
AccessionPrimary (citable) accession number: Q88VY1
Secondary accession number(s): F9UPM4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways