UniProtKB - Q88V90 (DAPF_LACPL)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Diaminopimelate epimerase
Gene
dapF
Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Functioni
Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation
Catalytic activityi
LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation
: L-lysine biosynthesis via DAP pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Diaminopimelate epimerase (dapF)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 13 | SubstrateUniRule annotation | 1 | |
Binding sitei | 73 | SubstrateUniRule annotation | 1 | |
Active sitei | 82 | Proton donorUniRule annotation | 1 | |
Binding sitei | 172 | SubstrateUniRule annotation | 1 | |
Sitei | 174 | Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation | 1 | |
Binding sitei | 209 | SubstrateUniRule annotation | 1 | |
Sitei | 227 | Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation | 1 | |
Active sitei | 236 | Proton acceptorUniRule annotation | 1 |
GO - Molecular functioni
- diaminopimelate epimerase activity Source: UniProtKB-EC
GO - Biological processi
- lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Isomerase |
Biological process | Amino-acid biosynthesis, Lysine biosynthesis |
Enzyme and pathway databases
BioCyci | LPLA220668:G1GW0-1867-MONOMER. |
UniPathwayi | UPA00034; UER00025. |
Names & Taxonomyi
Protein namesi | Recommended name: Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)Short name: DAP epimeraseUniRule annotation Alternative name(s): PLP-independent amino acid racemaseUniRule annotation |
Gene namesi | Name:dapFUniRule annotation Ordered Locus Names:lp_2185 |
Organismi | Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) |
Taxonomic identifieri | 220668 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149845 | 1 – 332 | Diaminopimelate epimeraseAdd BLAST | 332 |
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 220668.lp_2185. |
Structurei
3D structure databases
ProteinModelPortali | Q88V90. |
SMRi | Q88V90. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 83 – 84 | Substrate bindingUniRule annotation | 2 | |
Regioni | 227 – 228 | Substrate bindingUniRule annotation | 2 | |
Regioni | 237 – 238 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the diaminopimelate epimerase family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105E4Z. Bacteria. COG0253. LUCA. |
HOGENOMi | HOG000220466. |
KOi | K01778. |
OMAi | SMCGNGG. |
Family and domain databases
HAMAPi | MF_00197. DAP_epimerase. 1 hit. |
InterProi | View protein in InterPro IPR018510. DAP_epimerase_AS. IPR001653. DAP_epimerase_DapF. |
PANTHERi | PTHR31689. PTHR31689. 1 hit. |
Pfami | View protein in Pfam PF01678. DAP_epimerase. 2 hits. |
TIGRFAMsi | TIGR00652. DapF. 1 hit. |
PROSITEi | View protein in PROSITE PS01326. DAP_EPIMERASE. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q88V90-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVKMIKVHG SGNDFYLLDQ TQFQAPLSDA DLKQLAINIC KRDGAGLYDG
60 70 80 90 100
ADGVLVVDKS EHPQVLGRMR VINADGTEAS MCGNGLRTVA RYLGTQNSQE
110 120 130 140 150
DFRVQTMYAD LKVQAVADFA AHVPAYSVEI SPVTFDAQTL GMHANNDATT
160 170 180 190 200
IINEKIPALS ADLKFSAVAV PNPHLIAFVD HDTLVGPELG RIGEWMNDGK
210 220 230 240 250
NQIFPDGVNV SFVEVLGPNS IFVRTFERGV GFTNACGTAM SASSLMYVLL
260 270 280 290 300
HQESTDFNQE IHVTNPGGMV KTVVHQGADE EYWMELIGNA TFVRIVTLPL
310 320 330
EDALQGDYSP VTATETGEQV AYEDFVANLA KA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL935263 Genomic DNA. Translation: CCC79406.1. |
RefSeqi | WP_003644607.1. NC_004567.2. YP_004889920.1. NC_004567.2. |
Genome annotation databases
EnsemblBacteriai | CCC79406; CCC79406; lp_2185. |
GeneIDi | 1063577. |
KEGGi | lpl:lp_2185. |
PATRICi | fig|220668.9.peg.1847. |
Similar proteinsi
Entry informationi
Entry namei | DAPF_LACPL | |
Accessioni | Q88V90Primary (citable) accession number: Q88V90 Secondary accession number(s): F9UQB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2003 |
Last sequence update: | June 20, 2003 | |
Last modified: | March 28, 2018 | |
This is version 79 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |