Q88V90 (DAPF_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diaminopimelate epimerase
Short name=DAP epimerase
EC=5.1.1.7

Gene names

Name:	dapF
Ordered Locus Names:	lp_2185

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus plantarum

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197
Catalytic activity	LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00197.
Sequence similarities	Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	diaminopimelate epimerase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Diaminopimelate epimerase HAMAP-Rule MF_00197		PRO_0000149845
Regions
	Region	82 – 84	3	Substrate binding By similarity
	Region	227 – 228	2	Substrate binding By similarity
	Region	237 – 238	2	Substrate binding By similarity
Sites
	Active site	82	1	Proton donor/acceptor By similarity
	Active site	236	1	Proton donor/acceptor By similarity
	Binding site	13	1	Substrate By similarity
	Binding site	53	1	Substrate By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
	Site	174	1	Important for catalytic activity By similarity
	Site	227	1	Important for catalytic activity By similarity
Amino acid modifications
	Disulfide bond	82 ↔ 236		HAMAP-Rule MF_00197

Sequences

	Sequence		Length	Mass (Da)
	Q88V90 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 1D5FF6C6E05441E0	FASTA	332	35,962
	10 20 30 40 50 60 MAVKMIKVHG SGNDFYLLDQ TQFQAPLSDA DLKQLAINIC KRDGAGLYDG ADGVLVVDKS 70 80 90 100 110 120 EHPQVLGRMR VINADGTEAS MCGNGLRTVA RYLGTQNSQE DFRVQTMYAD LKVQAVADFA 130 140 150 160 170 180 AHVPAYSVEI SPVTFDAQTL GMHANNDATT IINEKIPALS ADLKFSAVAV PNPHLIAFVD 190 200 210 220 230 240 HDTLVGPELG RIGEWMNDGK NQIFPDGVNV SFVEVLGPNS IFVRTFERGV GFTNACGTAM 250 260 270 280 290 300 SASSLMYVLL HQESTDFNQE IHVTNPGGMV KTVVHQGADE EYWMELIGNA TFVRIVTLPL 310 320 330 EDALQGDYSP VTATETGEQV AYEDFVANLA KA

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL935263 Genomic DNA. Translation: CCC79406.1.
RefSeq	YP_004889920.1. NC_004567.2.
3D structure databases
ProteinModelPortal	Q88V90.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	220668.lp_2185.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC79406; CCC79406; lp_2185.
GeneID	1063577.
KEGG	lpl:lp_2185.
PATRIC	22250413. VBILacPla27411_1847.
Phylogenomic databases
eggNOG	COG0253.
HOGENOM	HOG000220466.
KO	K01778.
OMA	FILDQTE.
ProtClustDB	PRK00450.
Enzyme and pathway databases
UniPathway	UPA00034; UER00025.
Family and domain databases
HAMAP	MF_00197. DAP_epimerase.
InterPro	IPR018510. DAP_epimerase_AS. IPR001653. DAP_epimerase_DapF. [Graphical view]
PANTHER	PTHR31689:SF0. PTHR31689:SF0. 1 hit.
Pfam	PF01678. DAP_epimerase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00652. DapF. 1 hit.
PROSITE	PS01326. DAP_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPF_LACPL

Accession

Primary (citable) accession number: Q88V90
Secondary accession number(s): F9UQB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program