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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
Binding sitei600 – 6001ATPUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLPLA220668-WGS:GSPK-1868-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:lp_2187
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000432: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligasePRO_0000098403Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi220668.lp_2187.

Structurei

3D structure databases

ProteinModelPortaliQ88V89.
SMRiQ88V89. Positions 1-920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi597 – 6015"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88V89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKETLNLG KTKFKMRGNL PVKEVERQNV WAENKVYEQR QKLNEGKPSF
60 70 80 90 100
ILHDGPPYAN GDIHMGHALN KITKDFIVRY KSMNGFRAPY VPGWDTHGLP
110 120 130 140 150
IEQKLKQAGY DRKKMTDNEF RELCRKYALE QVDRQRDEFK RLGVAGEWDN
160 170 180 190 200
PYLTLKPEFE AAQVRVFGAF AKRHLIYRGQ KPVFWSWSSE SALAEAEVEY
210 220 230 240 250
HDVTSPSAFY GEHVIDGKGV LDDDTYMVVW TTTPWTIPAS EGITIDASFD
260 270 280 290 300
YVVVQPAGEN RKFVLAAELL DENAERFGWE DVQVLKTVKG AELENILCEH
310 320 330 340 350
PFDNSRHLVT MLGDFVTLDS GTGLVHTAPG YGEDDYRIGK QYDLPIFAPV
360 370 380 390 400
DDKGFLTAEA GDDFAGVFYD DANKIALDKL KAAGLLLKYM PYEHSYPFDW
410 420 430 440 450
RTKKPIIFRA TPQWFASVGD MRDEILKSMD DVEFFPEWGK KRLYNMIRDR
460 470 480 490 500
GDWVISRQRV WGVPLPIFYA EDGTAIMDEV TINHVADLFG KYGSNVWFER
510 520 530 540 550
DAKDLLPDGY TNEHSPNGTF TKETDIMDVW FDSGSSHQGV LAERSYLDYP
560 570 580 590 600
ADLYLEGSDQ YRGWFNSSLI TSVAVSGHAP YKQVLSQGFT LDNQGRKMSK
610 620 630 640 650
SLGNTIAPAD VIKQMGAEIV RMWVASVDTS SDVRVSMESF KQVADSYKKF
660 670 680 690 700
RNTVRFMLGN TADFDPKANR VAYEDLASVD QYMETRLNEF VAETKDHYDH
710 720 730 740 750
YDFLDIYKKL INFLTVDLSN FYLDIAKDIM YIDAEDSHSR RSMQTVFYDV
760 770 780 790 800
LVALTKLFTP MLPHTTEEIW PFLKEPEEFA QLAEMPAVQN YANSDELMAQ
810 820 830 840 850
WTRFMDLRSN VLKALEEARD AKLIGKSLEA HLDLYVDDDT QAFLDHLNTN
860 870 880 890 900
VRQMLMVSAL DLHALSDAPE DAETFGDTLA IKVGHADGDV CDRCRMVKTD
910 920 930
VGSDEAYPML CARCAAIVRE NYPESVTTGL EA
Length:932
Mass (Da):106,318
Last modified:June 1, 2003 - v1
Checksum:i559234B1D9AD149E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC79407.1.
RefSeqiYP_004889921.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC79407; CCC79407; lp_2187.
GeneIDi1063600.
KEGGilpl:lp_2187.
PATRICi22250415. VBILacPla27411_1848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC79407.1.
RefSeqiYP_004889921.1. NC_004567.2.

3D structure databases

ProteinModelPortaliQ88V89.
SMRiQ88V89. Positions 1-920.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220668.lp_2187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC79407; CCC79407; lp_2187.
GeneIDi1063600.
KEGGilpl:lp_2187.
PATRICi22250415. VBILacPla27411_1848.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.

Enzyme and pathway databases

BioCyciLPLA220668-WGS:GSPK-1868-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Entry informationi

Entry nameiSYI_LACPL
AccessioniPrimary (citable) accession number: Q88V89
Secondary accession number(s): F9UQB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: February 4, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.