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Q88V89

- SYI_LACPL

UniProt

Q88V89 - SYI_LACPL

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Protein

Isoleucine--tRNA ligase

Gene
ileS, lp_2187
Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylate By similarity
Binding sitei600 – 6001ATP By similarity
Metal bindingi891 – 8911Zinc By similarity
Metal bindingi894 – 8941Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi914 – 9141Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLPLA220668-WGS:GSPK-1868-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:lp_2187
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000432: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligaseUniRule annotationPRO_0000098403Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi220668.lp_2187.

Structurei

3D structure databases

ProteinModelPortaliQ88V89.
SMRiQ88V89. Positions 1-920.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi597 – 6015"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88V89-1 [UniParc]FASTAAdd to Basket

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MRVKETLNLG KTKFKMRGNL PVKEVERQNV WAENKVYEQR QKLNEGKPSF    50
ILHDGPPYAN GDIHMGHALN KITKDFIVRY KSMNGFRAPY VPGWDTHGLP 100
IEQKLKQAGY DRKKMTDNEF RELCRKYALE QVDRQRDEFK RLGVAGEWDN 150
PYLTLKPEFE AAQVRVFGAF AKRHLIYRGQ KPVFWSWSSE SALAEAEVEY 200
HDVTSPSAFY GEHVIDGKGV LDDDTYMVVW TTTPWTIPAS EGITIDASFD 250
YVVVQPAGEN RKFVLAAELL DENAERFGWE DVQVLKTVKG AELENILCEH 300
PFDNSRHLVT MLGDFVTLDS GTGLVHTAPG YGEDDYRIGK QYDLPIFAPV 350
DDKGFLTAEA GDDFAGVFYD DANKIALDKL KAAGLLLKYM PYEHSYPFDW 400
RTKKPIIFRA TPQWFASVGD MRDEILKSMD DVEFFPEWGK KRLYNMIRDR 450
GDWVISRQRV WGVPLPIFYA EDGTAIMDEV TINHVADLFG KYGSNVWFER 500
DAKDLLPDGY TNEHSPNGTF TKETDIMDVW FDSGSSHQGV LAERSYLDYP 550
ADLYLEGSDQ YRGWFNSSLI TSVAVSGHAP YKQVLSQGFT LDNQGRKMSK 600
SLGNTIAPAD VIKQMGAEIV RMWVASVDTS SDVRVSMESF KQVADSYKKF 650
RNTVRFMLGN TADFDPKANR VAYEDLASVD QYMETRLNEF VAETKDHYDH 700
YDFLDIYKKL INFLTVDLSN FYLDIAKDIM YIDAEDSHSR RSMQTVFYDV 750
LVALTKLFTP MLPHTTEEIW PFLKEPEEFA QLAEMPAVQN YANSDELMAQ 800
WTRFMDLRSN VLKALEEARD AKLIGKSLEA HLDLYVDDDT QAFLDHLNTN 850
VRQMLMVSAL DLHALSDAPE DAETFGDTLA IKVGHADGDV CDRCRMVKTD 900
VGSDEAYPML CARCAAIVRE NYPESVTTGL EA 932
Length:932
Mass (Da):106,318
Last modified:June 1, 2003 - v1
Checksum:i559234B1D9AD149E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL935263 Genomic DNA. Translation: CCC79407.1.
RefSeqiWP_011101679.1. NC_004567.2.
YP_004889921.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC79407; CCC79407; lp_2187.
GeneIDi1063600.
KEGGilpl:lp_2187.
PATRICi22250415. VBILacPla27411_1848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL935263 Genomic DNA. Translation: CCC79407.1 .
RefSeqi WP_011101679.1. NC_004567.2.
YP_004889921.1. NC_004567.2.

3D structure databases

ProteinModelPortali Q88V89.
SMRi Q88V89. Positions 1-920.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 220668.lp_2187.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CCC79407 ; CCC79407 ; lp_2187 .
GeneIDi 1063600.
KEGGi lpl:lp_2187.
PATRICi 22250415. VBILacPla27411_1848.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.

Enzyme and pathway databases

BioCyci LPLA220668-WGS:GSPK-1868-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Entry informationi

Entry nameiSYI_LACPL
AccessioniPrimary (citable) accession number: Q88V89
Secondary accession number(s): F9UQB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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