Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.By similarity

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-cysteine ligase activity Source: UniProtKB-EC
  3. glutathione synthase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLPLA220668-WGS:GSPK-2014-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:lp_2336
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000432 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 751751Glutathione biosynthesis bifunctional protein GshABPRO_0000192552Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi220668.lp_2336.

Structurei

3D structure databases

ProteinModelPortaliQ88UW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 336336Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.Curated

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000112828.
KOiK01919.
OMAiFNPSMYM.

Family and domain databases

InterProiIPR007370. Glu_cys_ligase.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88UW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDAVGKAI VQYHLVPLVH QANLGLEVTM HRVDAHGHLA TTAHPQAFGS
60 70 80 90 100
AQQNHQLRPG FSASALKFTT PVRRDIPALM AYLKGLNTAA RRSLDADERL
110 120 130 140 150
WPLSSTPVLP DDLTNVPLAD VDQVSYQRRR DLARKYELQR LMTTGSHVNM
160 170 180 190 200
SLNEALFTRL YTETFHQQYH SYVDFRNAIY LKVAQGLVRM NWLIQYLFGA
210 220 230 240 250
SPRLAVTDTT SRPQRSSVQH PDGRYSQVTG DYTSIDRYVA KLTAAVRQQQ
260 270 280 290 300
LLSVNDFDGP VRLRSNGQLA MMARQGVYYL EYRGLDLDPT SPVGVDANAV
310 320 330 340 350
AFVRLLASYF VMMPALPAKM VSQVNAQADQ LTRQVLGENP TTASAQAVPA
360 370 380 390 400
VQVLDALADF VKTYGLPNED AVLLKQLKSW VTDPKKTLSA QIAMQADPLA
410 420 430 440 450
WALERAARYQ ESSNERPFEL AGFTALDLSS QQLAQQALTR GVQVDVVDPH
460 470 480 490 500
ANILRLTKLG RSQLVVNGSG TDLNPQALTT VLTHKAAAKQ ILAEHGVPVP
510 520 530 540 550
ASQTYHTANQ LIADYDRYVQ AGGIVLKAAD ESHKVIVFRI MPERGLFEQV
560 570 580 590 600
VRQLFEQTSA VMAEEVVVAS SYRFLVIDSR VQAIVERIPA NIVGDGRSTV
610 620 630 640 650
KTLLDRKNGR ALRGTAFKWP QSALQLGTIE RYRLDSYHLT LDSVVSRGTQ
660 670 680 690 700
ILLREDATFG NGADVLDATA DMHQSYVQAV EKLVADLHLA VAGVDVMIPN
710 720 730 740 750
LYAELVPEHP EMAVYLGIHA APYLYPHLFP MFGTAQPVAG QLLDALFKNE

D
Length:751
Mass (Da):83,018
Last modified:June 1, 2003 - v1
Checksum:i605A494E7A7DCBF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC79530.1.
RefSeqiWP_011101718.1. NC_004567.2.
YP_004890044.1. NC_004567.2.

Genome annotation databases

EnsemblBacteriaiCCC79530; CCC79530; lp_2336.
GeneIDi1062357.
KEGGilpl:lp_2336.
PATRICi22250719. VBILacPla27411_1975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL935263 Genomic DNA. Translation: CCC79530.1.
RefSeqiWP_011101718.1. NC_004567.2.
YP_004890044.1. NC_004567.2.

3D structure databases

ProteinModelPortaliQ88UW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220668.lp_2336.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC79530; CCC79530; lp_2336.
GeneIDi1062357.
KEGGilpl:lp_2336.
PATRICi22250719. VBILacPla27411_1975.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000112828.
KOiK01919.
OMAiFNPSMYM.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.
BioCyciLPLA220668-WGS:GSPK-2014-MONOMER.

Family and domain databases

InterProiIPR007370. Glu_cys_ligase.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Entry informationi

Entry nameiGSHAB_LACPL
AccessioniPrimary (citable) accession number: Q88UW5
Secondary accession number(s): F9UQP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: April 1, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.