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Q88UW5 (GSHAB_LACPL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name=GCS-GS

Including the following 2 domains:

  1. Glutamate--cysteine ligase
    EC=6.3.2.2
    Alternative name(s):
    Gamma-ECS
    Short name=GCS
    Gamma-glutamylcysteine synthetase
  2. Glutathione synthetase
    EC=6.3.2.3
    Alternative name(s):
    GSH synthetase
    Short name=GS
    Short name=GSH-S
    Short name=GSHase
    Glutathione synthase
Gene names
Name:gshAB
Synonyms:gshF
Ordered Locus Names:lp_2336
OrganismLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]
Taxonomic identifier220668 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length751 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.

Catalytic activity

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Monomer By similarity.

Sequence similarities

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 751751Glutathione biosynthesis bifunctional protein GshAB
PRO_0000192552

Regions

Region1 – 336336Glutamate--cysteine ligase

Sequences

Sequence LengthMass (Da)Tools
Q88UW5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 605A494E7A7DCBF7

FASTA75183,018
        10         20         30         40         50         60 
MELDAVGKAI VQYHLVPLVH QANLGLEVTM HRVDAHGHLA TTAHPQAFGS AQQNHQLRPG 

        70         80         90        100        110        120 
FSASALKFTT PVRRDIPALM AYLKGLNTAA RRSLDADERL WPLSSTPVLP DDLTNVPLAD 

       130        140        150        160        170        180 
VDQVSYQRRR DLARKYELQR LMTTGSHVNM SLNEALFTRL YTETFHQQYH SYVDFRNAIY 

       190        200        210        220        230        240 
LKVAQGLVRM NWLIQYLFGA SPRLAVTDTT SRPQRSSVQH PDGRYSQVTG DYTSIDRYVA 

       250        260        270        280        290        300 
KLTAAVRQQQ LLSVNDFDGP VRLRSNGQLA MMARQGVYYL EYRGLDLDPT SPVGVDANAV 

       310        320        330        340        350        360 
AFVRLLASYF VMMPALPAKM VSQVNAQADQ LTRQVLGENP TTASAQAVPA VQVLDALADF 

       370        380        390        400        410        420 
VKTYGLPNED AVLLKQLKSW VTDPKKTLSA QIAMQADPLA WALERAARYQ ESSNERPFEL 

       430        440        450        460        470        480 
AGFTALDLSS QQLAQQALTR GVQVDVVDPH ANILRLTKLG RSQLVVNGSG TDLNPQALTT 

       490        500        510        520        530        540 
VLTHKAAAKQ ILAEHGVPVP ASQTYHTANQ LIADYDRYVQ AGGIVLKAAD ESHKVIVFRI 

       550        560        570        580        590        600 
MPERGLFEQV VRQLFEQTSA VMAEEVVVAS SYRFLVIDSR VQAIVERIPA NIVGDGRSTV 

       610        620        630        640        650        660 
KTLLDRKNGR ALRGTAFKWP QSALQLGTIE RYRLDSYHLT LDSVVSRGTQ ILLREDATFG 

       670        680        690        700        710        720 
NGADVLDATA DMHQSYVQAV EKLVADLHLA VAGVDVMIPN LYAELVPEHP EMAVYLGIHA 

       730        740        750 
APYLYPHLFP MFGTAQPVAG QLLDALFKNE D 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL935263 Genomic DNA. Translation: CCC79530.1.
RefSeqYP_004890044.1. NC_004567.2.

3D structure databases

ProteinModelPortalQ88UW5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING220668.lp_2336.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCC79530; CCC79530; lp_2336.
GeneID1062357.
KEGGlpl:lp_2336.
PATRIC22250719. VBILacPla27411_1975.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000112828.
KOK01919.
OMAPYIQTDF.
ProtClustDBPRK02471.

Enzyme and pathway databases

UniPathwayUPA00142; UER00209.
UPA00142; UER00210.

Family and domain databases

InterProIPR007370. Glu_cys_ligase.
[Graphical view]
PfamPF04262. Glu_cys_ligase. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHAB_LACPL
AccessionPrimary (citable) accession number: Q88UW5
Secondary accession number(s): F9UQP1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways