Q88UT1 (KITH_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	lp_2379

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus plantarum

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00124
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000174984
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	85 – 88	4	ATP By similarity
Sites
	Active site	86	1	Proton acceptor Potential
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	180	1	Zinc By similarity
	Metal binding	183	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88UT1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FB4E51939625CEBC	FASTA	192	22,147
	10 20 30 40 50 60 MAQLFFRYGA MNSGKTIEIL KVAHNYEEQD KSVIILTSGL DNRDGVGYVA SRIGLKREAT 70 80 90 100 110 120 PVFDDTNIFE IVKQTNPDAA CVLIDEAQFL KKHHVLELAD IVDELKIPVM TFGLKNDFRN 130 140 150 160 170 180 ELFEGSKYLL LYADKIEEMK TICWFCRKKA IMNLRFHDGQ PVYEGEQVQI GGNEAYYPVC 190 RHHYFYPPKL TK

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL935263 Genomic DNA. Translation: CCC79565.1.
RefSeq	YP_004890079.1. NC_004567.2.
3D structure databases
ProteinModelPortal	Q88UT1.
ModBase	Search...
Protein-protein interaction databases
STRING	220668.lp_2379.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC79565; CCC79565; lp_2379.
GeneID	1062425.
KEGG	lpl:lp_2379.
PATRIC	22250791. VBILacPla27411_2011.
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR027417. P-loop_NTPase. IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_LACPL

Accession

Primary (citable) accession number: Q88UT1
Secondary accession number(s): F9UQS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program