Q88U30 (PUR2_LACPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	lp_2719

Organism

Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) [Reference proteome] [HAMAP]

Taxonomic identifier

220668 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus plantarum

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151456
Regions
	Domain	109 – 309	201	ATP-grasp
	Nucleotide binding	135 – 190	56	ATP By similarity
Sites
	Metal binding	279	1	Magnesium or manganese By similarity
	Metal binding	281	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88U30 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 10AE0CDB704F08FF	FASTA	413	43,463
	10 20 30 40 50 60 MANVLVVGGG GREHAIAKAM MASPQVSTVY CAPGNPGMIR DGIRTLAIDE MDFAGLVNFA 70 80 90 100 110 120 RANTVTLTFV GPEVPLAAGI VDYFQAAGLA IFGPNQAAAQ LESSKVFAKA FMSRHHIPTA 130 140 150 160 170 180 DYRQFHELDS ALAYSHEQAV PQVIKVDGLA AGKGVTVATS HEQAAAAIKT AFKTSSTVLI 190 200 210 220 230 240 EDYVAGFEFS QMVLVGGEHY ALLPTAQDHK RLQDHDRGPN TGGMGAYSPV PQITPAVIDQ 250 260 270 280 290 300 TIATIIEPTI AGLKADGLSF EGVIYVGGIL TATGVQVIEY NLRLGDPETQ ILLPQLQSDF 310 320 330 340 350 360 YQVIVDLLQH RQPHAQWQTT ATYLGVVLAA PGYPGPTEAG VPVPTVAGAD YASVAGTPDQ 370 380 390 400 410 LVSAGGRVMM VTASAPSLVA AQRAVYQQID QLRVGDLVYR TDIGAKGMTA THD

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL935263 Genomic DNA. Translation: CCC79834.1.
RefSeq	YP_004890348.1. NC_004567.2.
3D structure databases
ProteinModelPortal	Q88U30.
ModBase	Search...
Protein-protein interaction databases
STRING	220668.lp_2719.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC79834; CCC79834; lp_2719.
GeneID	1063006.
KEGG	lpl:lp_2719.
PATRIC	22251333. VBILacPla27411_2274.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033463.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	CLSK2520478.
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LACPL

Accession

Primary (citable) accession number: Q88U30
Secondary accession number(s): F9URJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program