Reviewed, UniProtKB/Swiss-Prot Q88SV5 (GUAC_LACPL)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	lp_3271

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	GMP reductase HAMAP MF_01511		PRO_0000093760
Regions
	Nucleotide binding	205 – 228	24	NADP By similarity
Sites
	Active site	176	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88SV5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E19846BECA12DBE6	FASTA	325	35,432
	10 20 30 40 50 60 MGMEIFDYED IQLIPNKCVI NSRSEADTTV ELGGRRFKIP VVPANMATVI NDEIAKWLAT 70 80 90 100 110 120 NDYFYIMHRF APETRRAFIE TMHAAQLYAS ISVGVKSSEY DFIDDLATNQ IVPEYITIDI 130 140 150 160 170 180 AHGHADSVIA MIKHIKDVLP DSFVIAGNVA TPAAVRDLEN AGADATKVGV GPGKACITKV 190 200 210 220 230 240 KTGFGTGGWQ LAAVRWCAKA ARKPIIADGG VRTNGDIAKS IRFGATMVMI GSMLAGHQES 250 260 270 280 290 300 PGNILKIDGK TYKQYYGSAS ETQKGEHKNV EGKQMLVPYR GRLVDTLNEM QEDLQSSISY 310 320 AGGRDLKAIT KCDYVVVKNS IYNGD

References

[1]

"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL935261 Genomic DNA. Translation: CAD65404.1.
RefSeq	NP_786532.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3O based on UniProtKB P12268.
SMR	Q88SV5. Positions 5-321.
ModBase	Search...
Genome annotation databases
GeneID	1061982.
GenomeReviews	Gene locus lp_3271 in contig AL935263_GR.
KEGG	lpl:lp_3271.
NMPDR	fig|220668.1.peg.2666.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q88SV5.
OMA	NSRSECD.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_3271-MON.
BRENDA	1.7.1.7. 593.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_LACPL

Accession

Primary (citable) accession number: Q88SV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)