Q88RR3 (RSMB_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase B
EC=2.1.1.176

Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB

Gene names

Name:	rsmB
Synonyms:	rrmB, sun
Ordered Locus Names:	PP_0066

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA methylation Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Ribosomal RNA small subunit methyltransferase B		PRO_0000211800
Regions
	Region	251 – 257	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	373	1	Nucleophile Potential
	Binding site	275	1	S-adenosyl-L-methionine By similarity
	Binding site	301	1	S-adenosyl-L-methionine By similarity
	Binding site	320	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88RR3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3852A4A07B59FFCA	FASTA	436	47,451
	10 20 30 40 50 60 MNPRLAAARA LAAVLSGKAS LNSSLPAQLD KVDERDRGLT QDLAFGTARW QPRLDLLAAQ 70 80 90 100 110 120 LLQKPFKAAD ADVQALLLVG LYQLFYTRIP AHAAIGETVG CADKLKKPWA KGLLNAVLRR 130 140 150 160 170 180 AQREGEELLA GMERDPVVRT AHPRWLQKAL KAFWPEQWEA ICAANNAHPP MILRVNRRHH 190 200 210 220 230 240 SRDAYLALLA EAGIGASACQ YSRDGIVLAE ACDVRGLPGF AEGWVSVQDE AAQLSADLLE 250 260 270 280 290 300 LAPGQRVLDA CCAPGGKTCH LLEAEAGLAN MVAIDLEAKR LTRVRENLDR LQLDAELIAC 310 320 330 340 350 360 DARDTASWWD GKPFQRILLD APCSATGVIR RHPDIKMTRQ ADDIPALATL QGELLDALWP 370 380 390 400 410 420 TLEVGGMLLY ATCSTLPTEN TEVIKAFLAR TPGARELDLA TEAGLRQPHG RQLLAQEGGH 430 DGFYYAKLIK IAASRG

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN65700.1.
RefSeq	NP_742236.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88RR3.
ModBase	Search...
Protein-protein interaction databases
STRING	160488.PP_0066.
Proteomic databases
PRIDE	Q88RR3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN65700; AAN65700; PP_0066.
GeneID	1043547.
KEGG	ppu:PP_0066.
PATRIC	19938104. VBIPsePut30601_0071.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0144.
HOGENOM	HOG000037300.
KO	K03500.
OMA	KPPMWLR.
ProtClustDB	PRK10901.
Enzyme and pathway databases
BioCyc	PPUT160488:GIXO-66-MONOMER.
Family and domain databases
Gene3D	1.10.940.10. 1 hit.
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR018314. Fmu/NOL1/Nop2p_CS. IPR006027. NusB_RsmB_TIM44. IPR023267. RCMT. IPR004573. rRNA_ssu_MeTfrase_B. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 1 hit. PF01029. NusB. 1 hit. [Graphical view]
PRINTS	PR02008. RCMTFAMILY.
SUPFAM	SSF48013. NusB_RsmB_TIM44. 1 hit.
TIGRFAMs	TIGR00563. rsmB. 1 hit.
PROSITE	PS01153. NOL1_NOP2_SUN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMB_PSEPK

Accession

Primary (citable) accession number: Q88RR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program