Reviewed, UniProtKB/Swiss-Prot Q88R90 (GSH1_PSEPK)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:	gshA
Ordered Locus Names:	PP_0243

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Glutamate--cysteine ligase HAMAP MF_00578		PRO_0000192533

Sequences

	Sequence		Length	Mass (Da)
	Q88R90-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F868F5EC025D580B	FASTA	525	59,068
	10 20 30 40 50 60 MSELLNRRLS LLGANLPLLK QCLHGIEREC LRVTDEGRLA QTPHPEALGS ALTNEQITTD 70 80 90 100 110 120 YSESLLEFIT PALADPAKVL ESLEETHRFV YSKLGDEYLW SPSMPCTLPA EEDIPIAEYG 130 140 150 160 170 180 SSNIGKLKHV YRKGLALRYG RTMQCIAGIH YNFSLPEALW PLLREAEGST ENDRDYQSSA 190 200 210 220 230 240 YIALIRNFRR YSWLLMYLFG ASPALDKGFL RGRPHQLEEL DAETLFLPYA TSLRMSDLGY 250 260 270 280 290 300 QSNAQAGLTP CYNNLASYTD SLRKAVGTPY PPYVEIGTHV DGEWVQLNTN ILQIENEYYS 310 320 330 340 350 360 NIRPKRVTYT GERPIQALTS RGVQYVEVRC LDINPFLPVG IDLTEARFLD AFLLFCALED 370 380 390 400 410 420 SPQLDNGECG QCTSNFLTVV KEGRRPGLEL HRNGQPISLK DWASELIGRI RQLANLLDQA 430 440 450 460 470 480 QGSDEHAKAL DAQQAKVDDT SLTPSAQVLA RMTEHDESFV QFSLRQSRVH AETFREQPLS 490 500 510 520 NEKQQAFETL ARESLARQSE LEQNEVGDFD LFVGAYQASI LAISS

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN65875.1.
RefSeq	NP_742411.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1043812.
GenomeReviews	Gene locus PP_0243 in contig AE015451_GR.
KEGG	ppu:PP_0243.
NMPDR	fig|160488.1.peg.241.
TIGR	PP_0243.
Phylogenomic databases
HOGENOM	Q88R90.
OMA	Q88R90. DTLYLPY.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0243-MON.
Family and domain databases
HAMAP	MF_00578. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_PSEPK

Accession

Primary (citable) accession number: Q88R90

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)