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Protein

Probable alpha-L-glutamate ligase

Gene

rimK

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141ATPUniRule annotation1
Binding sitei187ATPUniRule annotation1
Metal bindingi248Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 2UniRule annotation1
Metal bindingi262Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179ATPUniRule annotation2
Nucleotide bindingi211 – 213ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-glutamate ligaseUniRule annotation (EC:6.3.2.-UniRule annotation)
Gene namesi
Name:rimKUniRule annotation
Ordered Locus Names:PP_0248
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002054731 – 301Probable alpha-L-glutamate ligaseAdd BLAST301

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_0248.

Structurei

3D structure databases

ProteinModelPortaliQ88R85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 287ATP-graspUniRule annotationAdd BLAST184

Sequence similaritiesi

Belongs to the RimK family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9I. Bacteria.
COG0189. LUCA.
HOGENOMiHOG000293092.
KOiK05844.
OMAiNYLRCYM.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01552. RimK. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88R85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAVLSRNP RLYSTRRLVE AGTQRGHEMV VIDTLRAYMN IASHKPQIHY
60 70 80 90 100
RGKPLEGFDA VIPRIGASVT FYGCAVLRQF EMMGVYPLNE SVAIARSRDK
110 120 130 140 150
LRSLQLLSRR GIGLPITGFA HSPDDIPDLI QMVNGAPLVI KVLEGTQGIG
160 170 180 190 200
VVLCETPQAA ESVIEAFMGL KQNIMVQEYI KEAGGADIRC FVVGDKVIAS
210 220 230 240 250
MKRQAKPGEF RSNLHRGGVA SLIKITPEER ITAIRAAKVM GLSVAGVDIL
260 270 280 290 300
RSNHGPLVME VNSSPGLEGI EVTTGKNVAG MIIEHLEKNG GPNQTRTKGK

G
Length:301
Mass (Da):32,629
Last modified:June 1, 2003 - v1
Checksum:i78894C76F30E69D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN65880.1.
RefSeqiNP_742416.1. NC_002947.4.
WP_003255817.1. NC_002947.4.

Genome annotation databases

GeneIDi1043821.
KEGGippu:PP_0248.
PATRICi19938508. VBIPsePut30601_0265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN65880.1.
RefSeqiNP_742416.1. NC_002947.4.
WP_003255817.1. NC_002947.4.

3D structure databases

ProteinModelPortaliQ88R85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_0248.

Protocols and materials databases

DNASUi1043821.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1043821.
KEGGippu:PP_0248.
PATRICi19938508. VBIPsePut30601_0265.

Phylogenomic databases

eggNOGiENOG4105D9I. Bacteria.
COG0189. LUCA.
HOGENOMiHOG000293092.
KOiK05844.
OMAiNYLRCYM.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01552. RimK. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIMK_PSEPK
AccessioniPrimary (citable) accession number: Q88R85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.