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Protein

Probable alpha-L-glutamate ligase

Gene

rimK

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141ATPUniRule annotation1
Binding sitei187ATPUniRule annotation1
Metal bindingi248Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 2UniRule annotation1
Metal bindingi262Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179ATPUniRule annotation2
Nucleotide bindingi211 – 213ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-270-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-glutamate ligaseUniRule annotation (EC:6.3.2.-UniRule annotation)
Gene namesi
Name:rimKUniRule annotation
Ordered Locus Names:PP_0248
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002054731 – 301Probable alpha-L-glutamate ligaseAdd BLAST301

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_0248

Structurei

3D structure databases

ProteinModelPortaliQ88R85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 287ATP-graspUniRule annotationAdd BLAST184

Sequence similaritiesi

Belongs to the RimK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9I Bacteria
COG0189 LUCA
HOGENOMiHOG000293092
KOiK05844
OMAiNYLRCYM

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_01552 RimK, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013651 ATP-grasp_RimK-type
IPR013815 ATP_grasp_subdomain_1
IPR023533 RimK
IPR004666 RpS6_RimK/Lys_biosynth_LsyX
PfamiView protein in Pfam
PF08443 RimK, 1 hit
TIGRFAMsiTIGR00768 rimK_fam, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q88R85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAVLSRNP RLYSTRRLVE AGTQRGHEMV VIDTLRAYMN IASHKPQIHY
60 70 80 90 100
RGKPLEGFDA VIPRIGASVT FYGCAVLRQF EMMGVYPLNE SVAIARSRDK
110 120 130 140 150
LRSLQLLSRR GIGLPITGFA HSPDDIPDLI QMVNGAPLVI KVLEGTQGIG
160 170 180 190 200
VVLCETPQAA ESVIEAFMGL KQNIMVQEYI KEAGGADIRC FVVGDKVIAS
210 220 230 240 250
MKRQAKPGEF RSNLHRGGVA SLIKITPEER ITAIRAAKVM GLSVAGVDIL
260 270 280 290 300
RSNHGPLVME VNSSPGLEGI EVTTGKNVAG MIIEHLEKNG GPNQTRTKGK

G
Length:301
Mass (Da):32,629
Last modified:June 1, 2003 - v1
Checksum:i78894C76F30E69D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN65880.1
RefSeqiNP_742416.1, NC_002947.4
WP_003255817.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN65880; AAN65880; PP_0248
GeneIDi1043821
KEGGippu:PP_0248
PATRICifig|160488.4.peg.265

Similar proteinsi

Entry informationi

Entry nameiRIMK_PSEPK
AccessioniPrimary (citable) accession number: Q88R85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health